中国兽医科学2018，Vol.48Issue(5)：658-663,6.DOI:10.16656/j.issn.1673-4696.2018.0075
闽西地区猪源大肠杆菌耐药基因blaCTX-M-9G的流行性分析
Investigation of blaCTX-M-9G resistance gene in Escherichia coli isolated from pig origin in the west of Fujian Province
摘要
Abstract
The aim of this research was to master the prevalence of antimicrobial resistance for β-lactam drugs and blaCTX-M-9G resistance gene in Escherichia coli isolated from pig origin in the west of Fujian, and provide guidance for the rational use of antibiotics in the locality. A total of 213 isolates collected from anus swab of diarrhea piglet were tested for antimicrobial susceptibility to β-lactam drugs by agar dilution method,and screened for resistance gene by PCR. It was found high resistance to ampicillin,amoxicillin/clavulanic acid,ceftiofur and cefotaxime,with resistance rates of 46.9％, 44.1％,28.6％ and 23.9％, respectively.17.4％ (37/213) isolates were positive for blaCTX-M-9G resistance gene,among those positive strains,they also were detected the PMQR genes,such as qnrA(5.4％ ),qnrB(37.8％ ), qnrS(73.0％ ),oqxA (70.3％ ),oqxB(75.7％ ) and qepA(18.9％ ),and 83.8％ isolates contained more than three kinds of resistance genes. The mostest prevalence genotypes was blaCTX-M-9G+oqxA+oqxB resistance genes,and its detection rate was 32.4％. Escherichia coli showed a certain resistance to β-lactam drugs in Western Fujian,and the resistance rate of blacCTX-M-9G resistance genes was quite high. A high rates of the PMQR genes were found among bla-CTX-M-9G positive strains.关键词
超广谱β-内酰胺酶/blaCTX-M/PMQR/耐药性Key words
extended-spectrum-β-actamases/blaCTX-M/PMQR/resistance分类
农业科技引用本文复制引用
杨守深,庄艺超,华雪妃,罗智玲,张杨英,吴德峰,杨小燕..闽西地区猪源大肠杆菌耐药基因blaCTX-M-9G的流行性分析[J].中国兽医科学,2018,48(5):658-663,6.基金项目
龙岩市科技计划项目(2015LY26) （2015LY26）
龙岩学院博士启动基金项目(LB2013001) （LB2013001）
