中国兽医科学2018，Vol.48Issue(6)：686-691,6.DOI:10.16656/j.issn.1673-4696.2018.0094
猪圆环病毒3型实时荧光定量PCR方法的建立和应用
Development and application of a real-time PCR for the detection of porcine circovirus 3
摘要
Abstract
Porcine circovirus 3(PCV3)is one of the novel virus found in pigs since 2016. Diagnosis of PCV3 is difficult with atypical clinical signs. The construct of a real-time PCR was described with a pair of special primers and a Taqman probe targeting a PCV3 genome in the GenBank. In order to rapidly detect PCV3, a real-time PCR assay was developed based on the PCV3 genome of the our country isolate, and a pair of primers and a Taqman probe, which were deisgned and synthesized by us. Some tests had proved this assay to be a optimal standard curve linearly with high specifity and sensitivity as well as good reproducibility. It's lowest detectable dilution was 10-8 (50 copies/ L)in the sensitivity test. For intra-batch and inter-batch reproducibility, the Cvvalues were repeated three times with an average value less than 3％. Additionally, 9 positive samples were tested from 36 field samples using this assay. In conclusion, this novel developed real-time PCR assay could be used as an efficient molecular biology tool to facilitate the diagnosis of PCV3.关键词
猪圆环病毒3型/实时荧光定量聚合酶链式反应/TaqMan 探针Key words
porcine circovirus 3(PCV3)/real-time PCR/TaqMan probe分类
农业科技引用本文复制引用
张志,郝占武,张美晶,吴发兴,刘爽,李晓成,王树双..猪圆环病毒3型实时荧光定量PCR方法的建立和应用[J].中国兽医科学,2018,48(6):686-691,6.基金项目
科技基础性工作专项(2012FY111000) （2012FY111000）
