中国兽医科学2018，Vol.48Issue(6)：707-711,5.DOI:10.16656/j.issn.1673-4696.2018.0115
猪细小病毒7型PCR检测方法的建立及应用
Establishment and application of PCRassay for the detection of porcine parvovirus 7
严美君 1杨涛涛 1李润成 1屈泰龙 1葛猛 1余兴龙1
作者信息
- 1. 湖南农业大学 动物医学院,湖南 长沙 410128
- 折叠
摘要
Abstract
Porcine parvovirus 7(PPV7) is a newly discovered parvovirus. To investigate the prevalence of PPV7 in pig herds, a pair primer was designed to specially amplify partial NS1 gene form the samples based on the PPV7 complete genome sequence in GenBank. The optimized annealing temperature of PCR reaction was preformed and its specificity and sensitivity were analyzed. The results showed that the target gene fragment could be specifically amplified from 52 to 72 ℃ in the annealing temperature range and the detection limit of the assay was 3. 6×102 copies/ L. 282 tissue samples, which were collected from different sources and 226 serum samples collected from four farms in Hunan Province, were evaluated by PCR and PPV7 was detected in 55. 0％(155/282)of the tissue samples and 10. 2％(23/226)of the serum samples. Notably, PPV7 had the highest prevalence rates in tissue samples of slaughter pigs(71. 4％), but whether PPV7 infection is associated with the health status of pigs remains unclear. This above-mentioned results provided foundation for the epidemiological study of PPV7.关键词
猪细小病毒7型/聚合酶链式反应/应用Key words
porcine parvovirus 7/PCR/application分类
农业科技引用本文复制引用
严美君,杨涛涛,李润成,屈泰龙,葛猛,余兴龙..猪细小病毒7型PCR检测方法的建立及应用[J].中国兽医科学,2018,48(6):707-711,5.
