中国现代医学杂志2018，Vol.28Issue(9)：1-5,5.DOI:10.3969/j.issn.1005-8982.2018.09.001
十二井穴放血对不同程度颅脑创伤大鼠脑水肿及线粒体生物合成的影响
Neuro-protective effect of blood-letting punctures at twelve Jing-Well points in rat model of traumatic brain injury
摘要
Abstract
Objective To investigate the effect of blood-letting punctures at twelve Jing-Well points on cerebral edema and mitochondrial biogenesis in rat model of traumatic brain injury (TBI). Methods A total of 56 male SD rats were randomly divided into 7 groups: mild TBI group, mild TBI + blood-letting group, moderate TBI group, moderate TBI + blood-letting group, severe TBI group, Severe TBI + blood-letting group and sham group. (8 for each group). TBI were conducted by controlled technic of cortical impact injury with impacting depth of 1mm (mild TBI), 3 mm (moderate TBI) and 4 mm (severe TBI). Rats in the sham group underwent the same operation except for cortical impacting. Blood-letting at Jing-Well points was performed by pricking the bilateral forelimb toes of the rats with 1ml syringe needle every 12 h with bleeding amount of 10 μl per point each time. The modified neurological severity score (mNSS) was recorded 72 h post operation. Brain tissue surrounding the lesion was harvested for analysis of PGC-1α, TFAM and mtDNA copy number. Brain water was measured. Results mNSS in 3 TBI groups were significantly higher compared with sham group, which increased along with the severity of TBI (P ＜ 0.05). Treatment of blood? letting decreased mild and moderate TBI-induced mNSS (P ＜ 0.05). Expression levels of PGC-1α,TFAM gene and the mtDNA copy in TBI group were significantly upregulated compared with Sham group (P ＜ 0.05). Treatment of blood? letting in mild TBI+blood-letting group aggrandized increase of mtDNA copy number when compared with TBI only groups (P ＜ 0.05). Expression of PGC-1α was upregulated by blood-letting therapy in moderate TBI group while no significant differences were observed in mild and severe TBI groups in terms of PGC-la and TFAM gene expressions (P ＞ 0.05). Water content in brain tissue of TBI group was significantly increased when compared with Sham group, which was decreased with the application of bloodletting therapy (P ＜ 0.05). Conclusion Neuroprotective effect of blood-letting punctures at twelve Jing-Well pointsis achieves probably due to the fact that it enhances energy supplement, ameliorates brain edema through activation of PGC mediated signal pathways and biosynthesis of mtDNA.关键词
颅脑创伤/十二井穴/线粒体DNA/神经功能损伤评分/放血疗法Key words
traumatic brain injury (TBI)/twelve jing-well points/mitochondrial dna/mNss/blood-letting therapy分类
医药卫生引用本文复制引用
沈彤,张赛,涂悦,陈旭义,吴焕成..十二井穴放血对不同程度颅脑创伤大鼠脑水肿及线粒体生物合成的影响[J].中国现代医学杂志,2018,28(9):1-5,5.基金项目
国家自然科学基金(No: 31200809 （）
11672332) （）
