中国现代医学杂志2018，Vol.28Issue(9)：22-27,6.DOI:10.3969/j.issn.1005-8982.2018.09.004
超声微泡介导沉默T淋巴细胞Itch基因对胃癌MFC细胞免疫杀伤作用的实验硏究
Effect of ultrasound-targeted microbubble destruction mediated silencing of Itch gene in T lymphocyte on cytotoxicity against MFC gastric cancer cells
刘丹 1张煜 1彭莉晴 1周静 1邹庆伟2
作者信息
- 1. 西南医科大学附属医院 超声科, 四川 泸州 646000
- 2. 西南医科大学附属医院 胃肠外科, 四川 泸州 646000
- 折叠
摘要
Abstract
Objective To investigate the effect of ultrasound-targeted microbubble destruction (UTMD) technology mediated silencing of Itch gene in T-lymphocytes on killing MFC gastric cancer cells. Methods T lymphocyte were isolated by magnetic beads. shRNA plasmid was constructed and transferred by UTMD technology to silence Itch gene in T lymphocytes. Twenty-four hours after transfection, the expression of CD69 was detected by flowcytometry; 48 hours after transfection, transfection efficiency and expression of Itch were identified by flowcytometry and Western blot, respectively; 72 hours after transfection, cytotoxicity activity against MFC gastric cancer cells were determined. Results Success rate of transfection by UTMD was 51.9％. Transfection of Itch increased expression of CD69 significantly when compared with remaining groups. Expression of Itch gene was sufficiently blocked by UTMD. Cytotoxicity of transfected T lymphocyte against MFC gastric cancer cells was dramatically upregulated when compared with negative control and blank group. Conclusion Silencing the expression of Itch gene by UTMD significantly promotes immune cytotoxicity of T lymphocyte against MFC gastric cancer cells in vitro.关键词
Itch/超声介导微泡破裂技术/基因沉默/胃癌/免疫治疗Key words
Itch/ultrasound-targeted microbubble destruction/gene silencing/gastric cancer/immunotherapy分类
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刘丹,张煜,彭莉晴,周静,邹庆伟..超声微泡介导沉默T淋巴细胞Itch基因对胃癌MFC细胞免疫杀伤作用的实验硏究[J].中国现代医学杂志,2018,28(9):22-27,6.
