中国现代医学杂志2018，Vol.28Issue(9)：28-32,5.DOI:10.3969/j.issn.1005-8982.2018.09.005
保护素DX对脓毒症小鼠急性肺损伤的保护作用
Protective effect of protectin DX on acute lung injury in mouse model of sepsis
卜克 1王璐 1刘林刚1
作者信息
- 1. 郑州大学第五附属医院, 河南 郑州 450052
- 折叠
摘要
Abstract
Objective To investigate the protective effect of protectin DX (PDX) on acute lung injury (ALI) in mouse model of sepsis. Methods Totally 45 healthy male Kunming mice were randomly divided into sham group, sepsis group and PDX group. Sepsis model was prepared with standard protocol. At the 60th min post surgery, mice in PDX group was intraperitoneally injected with 1μg of PDX while mice in sham group and sepsis group received the same amount of normal saline. At the 60th post surgery, lungs were harvested. The histopathological change of lung tissue was scored. Levels of interleukin-1β (IL-1β),interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-a),superoxide dismutase (SOD) and malondialdehyde (MDA) in lung tissue were measured. Nuclear factor-κB (NF-kB) in the nucleus and nuclear factor κ B inhibiting a antigen (IκB-α) in the cytoplasm of lung tissue were identified by Western blot. Results Compared with sham operation group, ALI the score were increased in the sepsis group, which was attenuated significantly by PDX (F = 253.323, P = 0.000). Levels of IL-1β, IL-6, TNF-α, SOD and MDA in the lung tissues of sepsis group were increased compared with sham group, which was dramatically ameliorated with treatment of PDX (P ＜ 0.05). Relative concentration of NF-κB in the nucleus upregulated while IκB-α in cytoplasm was down regulated in sepsis group when compared with sham group (P ＜ 0.05), which was significantly diminished by PDX therapy (P ＜ 0.05). Conclusion PDX protects sepsis inducecd lung injury through alleviating oxidative stress and inhibiting the NF-κB/IκB-α mediated release of inflammatory factors.关键词
PDX/脓毒症/急性肺损伤/小鼠Key words
pdx/sepsis/acute lung injury/mice分类
医药卫生引用本文复制引用
卜克,王璐,刘林刚..保护素DX对脓毒症小鼠急性肺损伤的保护作用[J].中国现代医学杂志,2018,28(9):28-32,5.
