中国现代医学杂志2018，Vol.28Issue(9)：33-39,7.DOI:10.3969/j.issn.1005-8982.2018.09.006
CNTF联合RA诱导成肌细胞类神经分化及与REST的相关性
CNTF combined with RA induces differentiation of myoblasts into neural like cells and its correlation with REST
摘要
Abstract
Objective To investigate methodological application of ciliary neurotrophic factor (CNTF) plus retinoic acid (RA) in neural like differentiation of myoblasts and its potential correlation with neuron restrictive silencing factor (REST). Methods Differentiation of C2C12 was induced by CNTF followed by neural like differentiation induced by RA. Cell morphology, immunofluorescence, flow cytometry analysis and Western blot were utilized to identify cellular type. Expression of REST pre and post neuronal differentiation was measured. Results Cells gathered as "neurospheres" after induction. Immunofluorescence staining showed expression of neural markers such as NSE, Soxl and GFAP. Neural like cells grew well after dispersed from "neurospheres" with high expression of nestin which is a neural stem cell biomarker. Western blot showed decreased expression of myoblast related markers such as Myogenin, P21, Desmin and REST post CNTF induction. Expression of neural associated markers including NSE, Soxl, GFAP was significantly increased. Flow cytometry data supported the upregulated expression of NSE in differentiated cells and NSE positive cell in differentiation group was about 84％. Conclusion CNTF combined with RA can induce differentiation of myoblasts into neural like cells, which is probably mediated by expression of REST.关键词
成肌细胞/睫状神经营养因子/视黄酸/神经元限制性沉默因子Key words
myoblast/CNTF/RA/rest分类
医药卫生引用本文复制引用
邱裕佳,马义勇,李光然,段思腾,李宇,王伟..CNTF联合RA诱导成肌细胞类神经分化及与REST的相关性[J].中国现代医学杂志,2018,28(9):33-39,7.基金项目
辽宁省自然科学基金(No: 201602322, 2015020556) （No: 201602322, 2015020556）
