中国现代医学杂志2018，Vol.28Issue(9)：40-44,5.DOI:10.3969/j.issn.1005-8982.2018.09.007
白藜芦醇阻断信号通路对鼻咽癌细胞生长的影响
Effect of Resveratrol on blocking growth of nasopharyngeal carcinoma cells
熊赪 1易阳艳2
作者信息
- 1. 江西省南昌市第一医院 耳鼻喉科, 江西 南昌 330000
- 2. 南昌大学第二附属医院 整形美容科, 江西 南昌 330000
- 折叠
摘要
Abstract
Objective To explore anticancer activity of Resveratrol in human nuclear pore complex cells (NPC). Methods MTT test was utilized to measure cellular growth of CNE-1 and CNE-2Z. Flow cytometry was performed to examine apoptosis and cell cycle status. Expression of several apoptosis associated proteins and cyclins was measured by Western blot. CNE-2Z xenografts on nude mice were performed to further validate anticancer capability of Resveratrol in vivo. Results Cell proliferation of NPC cells was inhibited by Resveratrol in time-dependent and dose-dependent manners, while apoptosis was significantly induced after being exposed to Resveratrol in dose-dependent manner. Flow cytometry results showed that Resveratrol arrestted NPC cells at the S-phases and G2/M- phases. Expression of Bcl-2 and hypoxia-inducible factor-la (HIF-la) were downregulated while caspase-3 as well as several cyclins involved in cell cycle regulation were upregulated with treatment of Resveratrol. Moreover, Resveratrol significantly decreased the phosphorylation levels of Akt, p70S6K and eukaryotic translation initiation factor 4E-binding protein-1 (4E-BP-1). In vivo study showed that Resveratrol dramatically inhibited the growth of NPC tumor xenografts. Conclusions Collectively, our findings suggest that Resveratrol exerts anti-proliferative and pro-apoptotic effects on human NPC cells through interfering with the Akt/p70S6K signaling pathways. 关键词
白藜芦醇/鼻咽癌/细胞增殖/细胞凋亡Key words
Resveratrol/nasopharyngeal carcinoma (NPC)/proliferation/apoptosis分类
医药卫生引用本文复制引用
熊赪,易阳艳..白藜芦醇阻断信号通路对鼻咽癌细胞生长的影响[J].中国现代医学杂志,2018,28(9):40-44,5.
