摘要
Abstract
Objective To analyze the diagnostic value of microRNA combined with TCT, HC2-HPV and pathology on early cervical cancer. Methods Totally 150 cases with suspected cervical cancer treated in our hospital from June 2014 to June 2015 were selected. The diagnostic value of TCT, HC2-HPV and micro RNA were analyzed based on pathological results. Results The diagnostic results of TCT (NR, ASCUS, ASCUS, LSIL, HSIL and SCC) were statistically different from those of pathology (BCC, CIN-I, CIN-II, CIN-III and SCC) (P ＜ 0.05). There were 73 cases with TCT diagnosis results ≥ ASCUS accounted for 48.67％ (73/150) and 79 cases with pathology ≥ CIN-I accounted for 52.67％ (79/150). The results of pathological diagnosis (BCC, CIN-I, CIN-II, CIN-III and SCC) and HC2-HPV positive rate were significantly different, and the difference gradually increased with severity of cervical cancer (χ2 = 23.7108, P = 0.000). The relative expression of pathological diagnosis was significantly associated with the results of microRNA. The expression levels of miRNA-21 and miRNA-193b were gradually increased with the severity of cervical lesions; while the expression levels of MiRNA-124 and miRNA-195 were gradually decreased with the severity of cervical cancer. The positive rate of HC2-HPV was increased gradually with the severity of cervical cancer, which was significant different (χ2 = 40.896, P = 0.000). The sensitivity of micro RNA combined with TCT and HC2-HPV was 98.73％, the specificity was 98.59％ and the diagnostic coincidence rate was 98.67％, which was significantly higher than that of microRNA, TCT or HC2-HPV only, respectively; or higher than microRNA combined with TCT, HC2-HPV combined with TCT or microRNA combined with HC2-HPV, respectively (P ＜ 0.05). In addition, the sensitivity, specificity and diagnostic accuracy of microRNAs were better than those of single TCT or HC2-HPV. Conclusion MicroRNA combined with TCT and HC2-HPV can be used for diagnosis of early cervical cancer, which is of high sensitivity, specificity and diagnostic coincidence rate.关键词
宫颈液基薄层细胞学检测/MicroRNA/二代杂交捕获法检测高危人乳头瘤病毒/早期宫颈癌/病理学Key words
TCT/micro RNA/HC2-HPV/early cervical cancer/pathology分类
医药卫生
