摘要
Abstract
Objectives To investigate the association of circulating E-cadherin (E-cad) and epidermal growth factor receptor (EGFR) with pathological behavior of breast cancer. Methods Totally 49 patients diagnosed with triple negative breast cancer (TNBC group), 198 patients diagnosed with non-triple negative breast cancer (NTNBC group) and 50 healthy subjects were involved in this study. Concentration of E-Cad and EGFR were obtained by enzyme-linked immunosorbent assay (ELISA) and the expression of FOXA1 was measured by immunohistochemistry. The clinical diagnostic value of serum E-cad and EGFR in breast cancer or TNBC was evaluated by ROC curve. The Youden index was utilized to evaluate the diagnostic threshold of E-cad and EGFR. Results Expression levels of E-Cad, EGFR and FOXA1 in breast cancer patients were significantly increased (P ＜ 0.01) compared with healthy volunteers. No statistically significant difference in E-Cad was observed between TNBC group and NTNBC group (P ＞ 0.05). Concentration of EGFR increased (P ＜ 0.01) while FOXA1 decreased (P ＜ 0.01) significantly in TNBC group when compared with NTNBC group. ROC curve showed that AUC of E-Cad and EGFR for breast cancer was 0.965 (95％ CI: 0.944, 0.986) and 0.758 (95％ CI: 0.686, 0.830), respectively. At the threshold of 1 010.7 ng/ml for E-Cad and 107.9 ng/ml EGFR, the Youden index was 0.807 and 0.385, sensitivity for diagnosis of breast cancer was 92.7％ and 78.5％, and the specificity was 88.0％ and 60％, respectively. AUC of EGFR for diagnosis of TNBC was 0.776 (95％ CI: 0.703, 0.848). At the threshold of 139.5 ng/ml, the largest Youden index was 0.473, the sensitivity was 79.6％, and the specificity was 67.7％. Conclusion E-cad and EGFR can be used as indicators of diagnosis for breast cancer while FOXA1 and EGFR can be a diagnostic candidate for diagnosis of TNBC.关键词
三阴性乳腺癌/E-钙黏蛋白/表皮生长因子受体/叉头框蛋白A1/受试者工作特征曲线Key words
triple-negative breast cancer/e-cadhenrin/epidermal growth factor receptor/fork head box protein a1/receiver operating characteristic curve分类
医药卫生
