转化医学杂志2018，Vol.7Issue(1)：20-26,7.DOI:10.3969/j.issn.2095-3097.2018.01.006
三氧可通过减轻小鼠脑小胶质细胞激活预防抑郁症发生
Ozone intraperitoneal injection prevents the depression by ameliorating microglia re-activation in depressed mice
摘要
Abstract
Objective Explore the impact of ozone on depression model, including the behaviors, the number of microglia cell and inflammation cytokines (IL-1β,IL-6 and TNF-α) in brain hippocampus, prefrontal cortex and amygdaloidal nucleus regions. Methods Forty mice were randomly divided into 4 groups; stress+saline (10), stress+ozone (10), control+saline (10), control + ozone (10). The model mice were set in a special centrifuge tube for 1 hour per day, lasting for a week, ozone by intraperitoneal injection of control + ozone and stress+ozone. Giving ozone 3 days before depression model as a ozone previously intervention. Giving ozone after depression model as a ozone therapeutic intervention. Tail suspension test (TST), forced swimming test (FST), novelty suppressed feeding test (NSFT), open field test (OFT) were subjected to detect the behavior of each group. Using the immunohistochemical technology to detect the number of microglia cell in brain hippocampus, prefrontal cortex and amygdaloidal nucleus regions. Using reverse transcription polymerase chain reaction technology to detect the expression level of inflammatory cytokines IL-1β,IL-6 and TNF-a from hippocampus, prefrontal cortex and amygdala region. Results After ozone previously intervention, ozone could improve depression behaviors in mice, reduce the number of activated microglial cells in the prefrontal cortex and hippocampus, decrease the levels of inflammatory cyto-kines IL-1β, TNF-α. After ozone intervention, ozone could not improve the depression behaviors in mice. There was no significant difference in the number of activated microglial cells in the prefrontal cortex, hippocampus and amygdala between stress+saline group and stress+ozone group (P＞0.05), ozone treatment failed to reduce the levels of IL-1β and TNF-a in the prefrontal cortex and hippocampus. Conclusion Ozone intraperitoneal injection has some effects on preventing the formation of depression, but there is no therapeutic effects on depression.关键词
三氧/抑郁/小胶质细胞/炎症因子/小鼠Key words
Ozone/Depression/Microglia cell/Inflammatory factors/Mice分类
医药卫生引用本文复制引用
芦国芳,安建雄,马钧,钱晓焱,王永,杨小荣..三氧可通过减轻小鼠脑小胶质细胞激活预防抑郁症发生[J].转化医学杂志,2018,7(1):20-26,7.基金项目
国家自然科学基金(81671076) （81671076）
国家青年科学基金(31000481) （31000481）
山西省自然科学基金(2011011040-2) （2011011040-2）
