转化医学杂志2018，Vol.7Issue(1)：27-30,4.DOI:10.3969/j.issn.2095-3097.2018.01.007
重组人粒细胞集落刺激因子对实验性自身免疫性脑脊髓炎小鼠的治疗作用
Therapeutic effect of rhG-CSF on experimental autoimmune encephalomyelitis in mice
摘要
Abstract
Objective The purpose of this study is to explore the therapeutic effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) on experimental autoimmune encephalomyelitis (EAE) mice. Methods Forty mice were randomly divided into 2 groups, and 10 in the control group were not treated, 30 in the model of EAE mice group induced by myelin oligodendrocyte glycoprotein 35-55, 6 mice were died, and the remaining 24 mice were randomly divided into model group and treatment group, with 12 mice in each group. The treatment group received rhG-CSF subcutaneously, and the model group received the same amount of saline. Neurological function score was evaluated during treatment. After 2 weeks of treatment, the brain and spinal cord specimens were taken for pathological observation and luxol fast blue (LFB) staining used for demyelination. The changes of the proportion of regulatory T (Treg) cells and natural killer (NK) cells were detected by flow cytometry. The expression of glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) in EAE mice were detected by Western blot. Results The results showed that the neurological function score, the inflammatory cell infiltration score and the demyelinating score of the rhG-CSF treatment group mice were reduced. The proportion of Treg cells in the treatment group were significantly lower than that in the model group, and the proportion of NK cells in treatment group were significantly lower than that in model group. Western blot showed that the expression of GFAP in the treatment group was significantly lower than that in the model group, whereas the expression of MBP was significantly higher than that in the model group. Conclusion rhG-CSF has a significant therapeutic effect on EAE.关键词
多发性硬化/自身免疫性脑脊髓炎/调节性T细胞/粒细胞集落刺激因子Key words
Multiple sclerosis/Autoimmune encephalomyelitis/Regulatory T cell/Granulocyte colony stimulating factor (G-CSF)分类
医药卫生引用本文复制引用
王震,樊心童,许春阳,张达矜,乔媛媛,李金凤,尹红蕾,段海峰,王运良..重组人粒细胞集落刺激因子对实验性自身免疫性脑脊髓炎小鼠的治疗作用[J].转化医学杂志,2018,7(1):27-30,4.基金项目
河南省2016年科技发展计划(162102310557) （162102310557）
