中国现代医生2018，Vol.56Issue(13)：28-32,5.
趋化因子CXCL12及CXCR4在子宫内膜癌组织中mRNA及蛋白表达水平临床研究
Clinical study of mRNA and protein expression level of chemokìnes CX-CL12 and CXCR4 in endometrial carcinoma
摘要
Abstract
Objective To provide a theoretical basis for the treatment of endometrial cancer by comparing the mRNA and protein expression levels of CXCL12 and CXCR4 in endometrial carcinoma. Methods A total of 100 patients admitted to the department from June 2014 to June 2017 were selected as the study subjects. Patients were at the age of 29-77 years, mean age(56. 8±9. 8) years. The expression of CXCL12 and CXCR4 was detected by tissue microarray, im-munohistochemistry and RT-PCR. Observed and recorded the patient's name, age and other basic information, recorded patient treatment efficacy. Results The positive expression rate of CXCL12 in endometrium group was 76. 8％, which was significantly higher than that in atypical endometrial hyperplasia group, simple endometrial hyperplasia group and normal endometrium group whose positive expression rates of CXCL12 were 18. 8％, 50. 0％, 35. 7％ respectively, the differences were statistically significant(P＜0. 05). The positive expression rate of CXCR4 in endometrium group was 71. 4％, which was significantly higher than that in atypical endometrial hyperplasia group, simple endometrial hyperplasia group and normal endometrium group whose positive expression rates of CXCR4 were 56. 3％、42. 9％、28. 6％ respectively, the differences were statistically significant^O. OS). The expression levels of CXCL12 mRNA were (0. 587± 0. 022) and (1. 132±0. 041) in the normal endometrium group and the endometrial carcinoma group respectively(P＜0. 05). The expression levels of CXCR4 mRNA were (0. 253±0. 035) and (0. 522±0. 258) in the normal endometrium group and endometrial cancer group respectively, and the difference was statistically significant (P＜0. 05). The expression of CX-CL12 was not related to the clinical stage and age of the tumor, and the difference was not statistically significant(P＞ 0. 05). The expression of CXCR4 was closely related to the clinical stage of tumor, and the difference was statistically significant (P＜0. 05). Conclusion The expression of CXCL12 and CXCR4 in patients with endometrial carcinoma was significantly increased. The expression of CXCR4 was closely related to the clinical stage of the tumor which provided accurate timing for the treatment of patients with endometrial cancer. At the same time, the majority of medical staff accumulated abundant experience from and these indicators can be widely applied.关键词
CXCL12/CXCR4/子宫内膜癌/mRNAKey words
CXCL12/CXCR4/Endometrial cancer/mRNA分类
医药卫生引用本文复制引用
陈晓璐,赵秀敏..趋化因子CXCL12及CXCR4在子宫内膜癌组织中mRNA及蛋白表达水平临床研究[J].中国现代医生,2018,56(13):28-32,5.基金项目
浙江省医药卫生科技计划基金资助项目(2015KYB429) （2015KYB429）
