中国现代医生2018，Vol.56Issue(15)：18-21,4.
NSCLC患者血清miR系列基因表达水平及与化疗疗效的相关性研究
Study on the correlation between the expression level of serum miRgene and chemotherapy efficacy in NSCLCpatients
摘要
Abstract
Objective To investigate the correlation between the expression levels of serum miR(microRNA) gene and chemotherapy efficacy in the patients with non-small cell lung cancer(NSCLC). Methods From March 2011 to September 2016, 200 NSCLCpatients without any anticancer treatment were selected as the NSCLCgroup. 100 healthy volunteers were selected as the healthy group. Serum samples were collected separately; serum samples before and after chemotherapy in 25 patients with advanced NSCLCwere collected. The qRT-PCRmethod was used to detect the expression level of serum miRgene in NSCLCgroup and healthy group, and the relationship between the expression level of serum miRgene and prognosis was determined. Results The serum levels of miR-19a, miR-19b-l, miR-20a, miR17-3p, miR-18a, and miR-92-1 in the NSCLCgroup were different from those in the healthy group(P＜0. 01). There were significant differences in the expressions of the above genes before and after chemotherapy in the serum of NSCLCpatients (P＜0. 05); the serum levels of miR-19a, miR-19b-l, miR-20a, miR-17-3p, miR-18a and miR-92-1 in the healthy group were significantly different from those before chemotherapy (P＜0. 05). There was no statistical difference compared with the patients after chemotherapy (P＞0. 05). Conclusion There is a high level of miRgene expression in the serum of NSCLCpatients. Chemotherapy reduces miRgene expression levels. The above six miRgene serum expressions contribute to the diagnosis, prediction and prognosis of NSCLC. The joint detection helps increase sensitivity.关键词
非小细胞肺癌/miR系列基因/预测/预后Key words
Non-small cell lung cancer (NSCLC)/miRgene/Prediction/Prognosis分类
医药卫生引用本文复制引用
陈群,石琴..NSCLC患者血清miR系列基因表达水平及与化疗疗效的相关性研究[J].中国现代医生,2018,56(15):18-21,4.基金项目
福建省自然科学基金资助项目(2014J01387) （2014J01387）
