植物学报2018，Vol.53Issue(3)：289-292,4.DOI:10.11983/CBB18023
中国科学家完成博落回全基因组测序并解析苄基异奎宁类生物碱途径
Chinese Scientists Sequenced the Genome and Dissected the Benzylisoquinoline Alkaloids Pathway of Macleaya cordata
张红艳 1罗杰2
作者信息
- 1. 华中农业大学园艺林学学院,武汉 430070
- 2. 华中农业大学生命科学技术学院,武汉 430070
- 折叠
摘要
Abstract
Macleaya cordata is a traditional Chinese medicinal plant species from the Papaveraceae family and is considered a safe resource of antimicrobial feed additive for livestock for its production of antimicrobial-active constituents such as sanguinarine (SAN) and chelerythrine (CHE). Chinese scientists reported the de novo whole-genome sequencing of M. cordata, the first to be sequenced from the Papaveraceae family. They then used the tissue-specific transcriptome and metabolic profiling data to identify 16 candidate genes involved in the biosynthesis of SAN and CHE. Homologous cloning and substrate-feeding experiments were then applied to verify the biochemical functions of the 14 candidates. This research set up the foundation for further improvement of the biosynthesis, regulation and biosynthetic chemistry of benzylisoquinoline alkaloids and also provides a powerful tool for the dissection of metabolic pathway(s) in other species from the Papaveraceae family.关键词
博落回/血根碱/白屈菜红碱/从头测序Key words
Macleaya cordata/sanguinarine/chelerythrine/de novo sequencing引用本文复制引用
张红艳,罗杰..中国科学家完成博落回全基因组测序并解析苄基异奎宁类生物碱途径[J].植物学报,2018,53(3):289-292,4.
