植物学报2018，Vol.53Issue(3)：313-321,9.DOI:10.11983/CBB17193
锁阳ITS序列遗传多样性分析
Analysis on Genetic Diversity of Cynomorium songaricum by ITS Sequence
任梦云 1杜乐山 2陈彦君 1张盾 1沈奇 2关潇 2张银东2
作者信息
- 1. 中国环境科学研究院,北京 100012
- 2. 海南大学热带农林学院,海口 570228
- 折叠
摘要
Abstract
To elucidate the genetic structure and genetic diversity of Cynomorium songaricum, modern molecular biology techniques at the DNA level were used to study the genetic structure of 188 C. songaricum individuals from 18 wild populations in the Hexi Corridor Region of Gansu and Qinghai. After alignment, all amplified sequence lengths were 687 bp. The 687 bp ITS sequence detected 7 mutation sites in 188 individuals,defining 9 haplotypes. Processing these 9 haplotype sequences led to a data matrix for calculating the haplotype diversity (Hd=0.294 20) and nucleotide diversity (π=0.000 49). In the haplotype network map, H1 is located in the center and distributed in all populations, and is the ancient and core haplotype. AMOVA revealed that the variation in C. songaricum mainly occurs in populations. According to the genetic differentiation coefficient and Mantel test of ITS sequences, we found no significant relation between genetic and geographic distances, so the current distribution of C. songaricum represents the fragmentation product in recent time. Detection of historical expansion of populations showed that the Tajima's D test rejected a neutral mutation evolution, and the population expansion history or gene locus is under negative selection pressure, so the null hypothesis cannot be ruled out. Our study provides molecular evidence for the classification system, identification and protection measures of C. songaricum.关键词
锁阳/ITS/遗传多样性/中性假说Key words
Cynomorium songaricum/ITS/genetic diversity/neutral hypothesis引用本文复制引用
任梦云,杜乐山,陈彦君,张盾,沈奇,关潇,张银东..锁阳ITS序列遗传多样性分析[J].植物学报,2018,53(3):313-321,9.
