植物学报2018，Vol.53Issue(3)：334-340,7.DOI:10.11983/CBB17207
怀山药类原球茎的诱导形成与植株再生
Induction and Plant Regeneration of Protocorm-like Bodies in Dioscorea opposita
摘要
Abstract
To improve the speed of in vitro propagation of Dioscorea opposita and shorten the breeding cycle, we used nodal segments with axillary buds of D. opposita cv. 'Tiegun' as the starting material. The optimal conditions for protocorm-like body (PLB) induction, proliferation, differentiation and plantlet regeneration were analyzed. The optimal culture medium for PLB induction was MS+1.0 mg·L-1 TDZ+30 g·L-1 sucrose, the optimal culture medium for PLB proliferation was MS+9 mg·L-1 6-BA+30 g·L-1 sucrose, and the optimal culture medium for PLB differentiation was MS+2 mg·L-1 KT+0.02 mg·L-1 NAA+30 g·L-1 sucrose. In culture medium containing 1/4MS+0.05 mg·L-1 NAA+1.0 mg·L-1 PP333+15 g·L-1 sucrose, the rooting rate of regenerated plantlets from PLBs was 80％, and the survival rate of regenerated plantlets was up to 85％ after being sown in soil. The induction of protocorm like bodies and the establishment of plant regeneration system provide a new way for rapid propagation of D. opposita seedlings.关键词
铁棍山药/诱导/植株再生/增殖/类原球茎Key words
Dioscorea opposita/induction/plant regeneration/proliferation/protocorm-like body引用本文复制引用
李瑞雪,李纪强,蒲腾飞,张晓丽,赵喜亭,李俊华,李明军..怀山药类原球茎的诱导形成与植株再生[J].植物学报,2018,53(3):334-340,7.基金项目
国家自然科学基金(No.81274019)、国家中医药管理局中医药行业科研专项子课题(No.201407005-08)和河南省创新型科技人才队伍建设工程(No.C20130037) （No.81274019）
