植物学报2018，Vol.53Issue(3)：341-352,12.DOI:10.11983/CBB17103
淫羊藿生物碱的超声波-微波协同提取及其对HeLa细胞的抑制作用
Ultrasound-microwave-assisted Extraction of Alkaloids from Epimedium brevicornum and Their Inhibitory Effect on HeLa Cells
摘要
Abstract
A protocol of ultrasound-microwave-assisted extraction was developed to isolate alkaloids from the medicinal plant Epimedium brevicornum, and the extraction mechanism is discussed. The chemical composition of the alkaloid extract was analyzed. The inhibitory effect of E. brevicornum alkaloids on human cervical cancer HeLa cells was investigated. The optimal condition for ultrasound-microwave-assisted extraction of E. brevicornum alkaloids was achieved by using an orthogonal test: steeped time, 40 min; ultrasound-microwave time, 18 min; microwave power, 250 J·s-1; liquid-to-solid ratio, 30 mL·g-1; and ethanol concentration, 70％. Extraction yield of alkaloids by ultrasound- microwave-assisted extraction reached 16.146 mg·g-1, which was significantly higher than that by ultrasound-assisted extraction, microwave-assisted extraction and heating extraction. Scanning electron micrographs showed that the synergism between the ultrasound and microwave resulted in many interstices on the surface of leaf samples of E, brevicornum. Laser diffraction particle sizing analysis revealed that the amount of small and medium particles of leaf samples increased and that of large particles decreased after ultrasound-microwave-assisted extraction. Thin-layer chromatography and high-performance liquid chromatography revealed that the alkaloid extract contained magnoflorine, and berberine was not detectable. E. brevicornum alkaloids exhibited a significant dose-dependent inhibitory activity against human cervical cancer HeLa cells. The present research may be helpful for the exploitation and utilization of Epimedium resources.关键词
心叶淫羊藿/生物碱/超声波-微波协同提取/机理/HeLa细胞Key words
Epimedium brevicornum/alkaloids/ultrasound-microwave-assisted extraction/mechanism/HeLa cells引用本文复制引用
李璐,安叶娟,乔春雷,杨晓华,张华峰,薛梦莹..淫羊藿生物碱的超声波-微波协同提取及其对HeLa细胞的抑制作用[J].植物学报,2018,53(3):341-352,12.基金项目
中央高校基本科研业务费专项资金(No.GK2016CSZ008, No.GK201703076)、陕西省科技合作项目(No.2014SJ-01, No.2014SJ08)和陕西省科学技术研究发展计划(No.2016SF-350) （No.GK2016CSZ008, No.GK201703076）
