南京医科大学学报（自然科学版）2018，Vol.38Issue(5)：648-652,5.DOI:10.7655/NYDXBNS20180515
LC-MS/MS法测定人尿液中法罗培南的浓度及其尿药排泄研究
Determination of faropenem in human urine by a LC-MS/MS method and study on urinary excretion
摘要
Abstract
Objective:To establish a LC-MS/MS method for the determination of faropenem in human urine, and to investigate its urinary excretion after intravenous administration of faropenem in healthy volunteers. Methods:The hydrochlorothiazide was using as the internal standard. Chromatographic separation was carried on a Waters Cortecs C18 (2. 1 mm × 50 mm, 2. 7 μm). The mobile phase was consisted of acetonitrile and 0. 5％ formic acid (10∶90). Electrospray ionization source was applied and operated in the negativeion mode using multiple reaction monitoring. Urinary excretion parameters were calculated after single and multiple intravenous administration of faropenem at 300 mg and 400 mg to healthy volunteers. Results:The calibration curve was linear in the range of 0. 506-506. 000 μg/m L. The accuracy, inter-and intra-precision were all below 15％. The matrix effect did not affect the determination results. Analytes were stable during the study. The average cumulative excretion and average cumulative excretion rates of faropenem in healthy volunteers after single intravenous infusion of 300 mg were (65. 16 ± 43. 63) mg and (21. 7 ± 14. 5)％, respectively. Conclusion:This method is suitable for the determination of faropenem in human urine.关键词
法罗培南/LC-MS/MS/尿药排泄Key words
faropenem/LC-MS/MS/urinary excretion分类
医药卫生引用本文复制引用
吴德芹,孙鲁宁,钱依,方云茜,张宏文,童明庆,陈安九,王永庆..LC-MS/MS法测定人尿液中法罗培南的浓度及其尿药排泄研究[J].南京医科大学学报（自然科学版）,2018,38(5):648-652,5.基金项目
国家自然科学基金(81503160,81673515) （81503160,81673515）
江苏省"六大人才高峰"项目(2014-YY-001) （2014-YY-001）
