兽类学报2018，Vol.38Issue(3)：235-246,12.DOI:10.16829/j.slxb.150194
粪便DNA分析技术在分子生态学研究中的机遇与挑战
Opportunities and challenges of fecal DNA technology in molecular ecology researches
摘要
Abstract
Advances in fecal DNA technology have expanded its usages in molecular ecology,especially in genetic assessment of wild animals. The technology allows researchers to understand ecological issues without contacting,disturbing,or even seeing animals,thus avoided invasions to the animal studied,and greatly promotes studies in molecular ecology of wild animals. Although this technology could yield poor DNA and relatively high genotyping errors in its early stages,these problems have been overcome gradually with the technological achievements being made in the past 25 years. Nowadays, fecal DNA technology yields good DNA and low genotyping errors,allowing researchers to address questions in reliability. Here,we share our knowledge about technological pitfalls on fecal sampling,preservation,DNA extraction,PCR,and genotyping in detail,and discuss opportunities and challenges of its applications,aiming to increase the power and role of the technology in molecular ecology of wild animals.关键词
粪便DNA/非损伤性取样/野生动物/分子生态学/微卫星分型Key words
Fecal DNA/Microsatellite genotyping/Molecular ecology/Noninvasive sampling/Wild animals分类
生物科学引用本文复制引用
单磊,胡义波,魏辅文..粪便DNA分析技术在分子生态学研究中的机遇与挑战[J].兽类学报,2018,38(3):235-246,12.基金项目
科技部重点研发计划项目(2016YFC0503200) （2016YFC0503200）
国家自然科学基金项目(31670386) （31670386）
