生命科学研究2018，Vol.22Issue(2)：99-113,15.DOI:10.16605/j.cnki.1007-7847.2018.02.003
16个Y-STR基因座快速复合扩增体系的构建研究
Study on Construction of a 16-Plex Rapid Y-STR Amplification System
摘要
Abstract
To establish a 16-plex rapid Y-STR amplification system for the actual needs of public security, sixteen STR loci, including DYS19, DYS385a/b, DYS390, DYS391,DYS392, DYS393,DYS438, DYS439, DYS437, DYS448, DYS456, DYS458, DYS635, Y_GATA H4 and DYS447, were chosen based on the current Y-STR genotyping for development of the rapid PCR. Roche FastStart Taq DNA Polymerase and DNA standard sample 9948 were used during an amplification and optimization process. The 16-plex rapid Y-STR amplification system was achieved by performing various experiments, including choosing amplification conditions and the volume of DNA polymerase, and adjusting interlocus balance, thermal cycling parameters and the volume of rapid PCR system, reaction buffers and a lot of additives. The results showed that, through this 16-plex rapid Y-STR amplification system, all 16 samples of Y-STR loci could be obtained in 30 minutes, with a good balance between the alleles and without nonspecific amplification. It proved that the rapid recombination amplification system of 16 Y-STR sites can improve the efficiency of sample detection and is of practical significance for the timeliness of special cases in public security.关键词
法医DNA/快速PCR/快速Y-STR复合扩增/16个基因座/遗传多态性Key words
forensic DNA/rapid PCR/rapid Y-STR multiplex amplification/16 loci/genetic polymorphism分类
生物科学引用本文复制引用
董倩,叶健,朱波峰,孙敬,欧元,韩俊萍,李彩霞,尚蕾,赵蕾,丁光树,孙辉,李万水..16个Y-STR基因座快速复合扩增体系的构建研究[J].生命科学研究,2018,22(2):99-113,15.基金项目
"十三五"国家重点研发计划项目(2017YFC0803507) （2017YFC0803507）
公安部技术研究计划项目(2016JSYJC11) （2016JSYJC11）
本研究生物样本来源于国家人类遗传资源共享服务平台(YCZYPT[2017]01-3, Biological samples were provided by NICGR). （YCZYPT[2017]01-3, Biological samples were provided by NICGR）
