生命科学研究2018，Vol.22Issue(2)：129-135,7.DOI:10.16605/j.cnki.1007-7847.2018.02.006
高脂高糖食物诱导中缅树鼩肥胖的研究
Research on High-fat and High-sugar Diet-induced Obesity in Tupaia belangeri
摘要
Abstract
In order to investigate the high-fat and high-sugar diet-induced obesity model in Tupaia belangeri, blood index measurement, flow cytometry analysis and positron emission tomography/computed tomography (PET/CT) scanning on adipose tissue of T. belangeri were performed under the condition of high-fat and high-sugar food in the present study. The results showed that the mass of white adipose tissue (WAT) was 3.7 times heavier than that of the control group on day 28,and was 6.4 times heavier than that of the control group on day 49. The triglyceride (TG) and high density lipoprotein (HDL) levels were significantly higher than those of the control group on day 49,and the blood glucose levels, total cholesterol (CHOL) and blood platelet(PLT) levels showed extremely significant differences between experimental group and control group on day 49. Sixty minutes after absorption of 18-fluoro-fluorodeoxyglucose (18F-FDG) in WAT and brown adipose tissue(BAT), the standardized uptake value (SUV) was significantly higher than that of the control group on day 28. But on day 49,the SUV declined. Flow cytometry analysis showed that positive markers of R2 group in WAT and BAT increased significantly on day 28, and decreased on day 49. All of the results showed that high-fat and high-sugar food could induce obesity and increase the mass of WAT significantly in T. belan-geri. The obesity resistance phenomenon may appear on day 28, but with the extension of acclimation time, it will gradually reduce.关键词
中缅树鼬/脂肪组织/流式细胞术/正电子发射计算机断层显像/高糖高脂食物/肥胖Key words
Tupaia belangeri/adipose tissue/flow cytometry/positron emission tomography/computed tomography (PET/CT)/high-fat and high-sugar diet/obesity分类
生物科学引用本文复制引用
朱万龙,侯东敏,左木林,王政昆..高脂高糖食物诱导中缅树鼩肥胖的研究[J].生命科学研究,2018,22(2):129-135,7.基金项目
国家自然科学基金资助项目(31660121) （31660121）
云南省科技计划重点项目(2016FA045) （2016FA045）
