生命科学研究2018，Vol.22Issue(2)：136-142,7.DOI:10.16605/j.cnki.1007-7847.2018.02.007
潞党参多糖的抗补体活性分析
Anti-complementary Activity of Polysaccharides from Lu Codonopsis pilosula
摘要
Abstract
The anti-complementary activity of polysaccharides from Lu Codonopsis pilosula (LCPP) was inves-tigated in this paper. The hemolysis assay was used to evaluate the effects of LCPP on the classical and alternative pathways of complement activation. It proved that LCPP repressed the activity of complementary system in a dose -dependent manner, and the CH50 and AP50 values were 2.061 ±0.127 mg/mL and 6.725± 0.895 mg/mL, respectively. Furthermore, real-time PCR was used to assess the effect of LCPP on comple-ment component C3 expression in human HepG2 cells. The results showed that LCPP suppressed C3 expres-sion in TNF-a-mediated HepG2 cells significantly. Therefore, Lu Codonopsis pilosula is a potential anti-complementary agent, and may be used in the treatment of autoimmune diseases.关键词
潞党参/多糖/补体系统/抗补体/细胞溶血实验/实时定量PCRKey words
Lu Codonopsis pilosula/polysaccharides/complement system/anti -complement/hemolysis assay/real-time PCR分类
医药卫生引用本文复制引用
李平,胡建燃,史宝忠..潞党参多糖的抗补体活性分析[J].生命科学研究,2018,22(2):136-142,7.基金项目
山西省高校创新学科群项目子项目(XKQZXM200601) （XKQZXM200601）
山西省重点学科建设经费资助项目 （）
