中国药理学通报2018，Vol.34Issue(9)：1226-1230,5.DOI:10.3969/j.issn.1001-1978.2018.09.009
酪氨酸羟化酶与钩藤碱抑制甲基苯丙胺依赖小鼠条件性位置偏爱的关系研究
Effect of rhynchophylline on expression of TH in methamphetamine-induced CPP mice
摘要
Abstract
Aim To explore the effect of rhynchophyl-line on the expression of tyrosine hydroxylase ( TH) in hippocampus of methamphetamine-induced condition place preference ( CPP) mice. Methods Metham- phetamine was injected intraperitoneally to mice, and the expression of TH was observed by immunohisto-chemistry and Western blot. Results The CPP mouse model was established successfully by methamphet-amine ( 4 mg·kg-1) . Ketamine ( 15 mg·kg-1) , rhynchophylline low dosage group (40 mg·kg-1) and rhynchophylline high dosage group ( 80 mg·kg-1) could remove the effect of methamphetamine on CPP mice. The result of immunohistochemistry showed that methamphetamine ( 4 mg·kg-1) could increase the number of TH positive cells in hippocampus while ket-amine (4 mg·kg-1), rhynchophylline (40, 80 mg· kg-1) group could attenuate the change. Western blot-ting indicated the expression of TH of model group in-creased significantly, whereas ketamine ( 15 mg· kg-1) , rhynchophylline ( 40, 80 mg·kg-1) group presented less expression. Conclusions The CPP in-duced by methamphetamine in mice may be inhibited to some extent by rhynchophylline, and its mechanism may be associated with the expression of TH.关键词
钩藤碱/甲基苯丙胺/条件性位置偏爱/药物依赖/酪氨酸羟化酶/氯胺酮Key words
rhynchophylline/methamphetamine/con-dition place preference/drug addiction/tyrosine hydroxylase/katamine引用本文复制引用
朱晨,刘伟,李璟,李婵,房淼,陈志杰,周玉婷,罗超华,莫志贤..酪氨酸羟化酶与钩藤碱抑制甲基苯丙胺依赖小鼠条件性位置偏爱的关系研究[J].中国药理学通报,2018,34(9):1226-1230,5.基金项目
国家自然科学基金资助项目(No 81673628, 81573706) （No 81673628, 81573706）
