中国药理学通报2018，Vol.34Issue(9)：1253-1257,5.DOI:10.3969/j.issn.1001-1978.2018.09.014
淫羊藿次苷Ⅱ通过调节MMP-2、MMP-9和TIMP-1的表达抗自发性高血压大鼠心肌纤维化
Icariside Ⅱ ameliorates myocardial fibrosis in spontaneously hypertensive rats through regulating expression of MMP-2, MMP-9 and TIMP-1
摘要
Abstract
Aim To investigate the effects of IcarisideⅡ ( ICS Ⅱ) on myocardial fibrosis in spontaneously hypertensive rat( SHR) . Methods Twenty male SHR rats were randomly divided into the model group (group SHR) , ICS Ⅱ low ( ICS Ⅱ-L) , middle ( ICSⅡ-M) and high ( ICS Ⅱ-H) group, and male WKY rats were set as control group ( group WKY) . ICS Ⅱ-L, ICSⅡ-M and ICSⅡ-H groups were intragastrically administered with ICS Ⅱ for 12 weeks. After that the blood pressure was measured in rats. Then, the rats were sacrificed and the left ventricles were separated in order to calculate the left ventricular mass index. Mas-son staining was used to detect the occurrence of inter-stitial fibrosis in cardiac tissues. Real time PCR was used to observe the gene expression of MMP-2, MMP-9 and TIMP-1 in the left ventricle in SHRs. The protein expression levels of MMP-2, MMP-9, TIMP-1, Colla- gen Ⅰ and Collagen Ⅲ were measured by Western blot. Results Compared with SHR group, the myo-cardial fibrosis was reduced after ICS Ⅱ (8, 16 mg· kg-1) treatment. The blood pressure and left ventricu-lar mass index decreased(P<0.05). The expressions of MMP-2, MMP-9, CollagenⅠand CollagenⅢwere down-regulated in left ventricular tissues( P <0.05 ) , while the expression of TIMP-1 was up-regulated( P<0.05) . Conclusion Icariside Ⅱ ameliorates myocar-dial fibrosis in SHR, and the mechanisms might be re-lated to the decrease of blood pressure and down-regu-lation of MMP-2, MMP-9 expression and up-regulation of TIMP-1 expression.关键词
淫羊藿次苷Ⅱ/心肌纤维化/自发性高血压大鼠/MMP-2/MMP-9/TIMP-1/细胞外基质Key words
icariside Ⅱ/myocardial fibrosis/sponta-neously hypertensive rat/MMP-2/MMP-9/TIMP-1/extracellular matrixc引用本文复制引用
付舒,李叶丽,吴雨婷,岳云,高杨,杨丹莉..淫羊藿次苷Ⅱ通过调节MMP-2、MMP-9和TIMP-1的表达抗自发性高血压大鼠心肌纤维化[J].中国药理学通报,2018,34(9):1253-1257,5.基金项目
国家自然科学基金专项基金(No 81660679) （No 81660679）
