中国药理学通报2018，Vol.34Issue(9)：1296-1301,6.DOI:10.3969/j.issn.1001-1978.2018.09.021
桑白皮多酚对B16细胞内黑色素生成的影响及其机制
Effect of polyphenol from Cortex Mori on melanogenesis of B16 cells and its mechanism
摘要
Abstract
Aim To investigate the inhibitory effect of polyphenol from Cortex Mori( CMP) on melanogenesis in mouse melanoma B16 cells and its possible mecha- nism. Methods Melanoma B16 cells with high ex-pression melanin were induced by α-melanocyte-stimu-lating hormone ( α-MSH) to establish cell model. Cell viability was detected by MTT assay. The melanin syn-thesis and tyrosinase activity were measured by NaOH and L-Dopa assays, respectively. The tyrosinase (TYR), tyrosinase-related protein-1 (TRP-1), tyrosi-nase-related protein-2 ( TRP-2 ) and microphthalmia associated transcription factor ( MITF ) protein and mRNA levels were measured by Western blot and qRT-PCR, respectively. Results CMP could inhibit the melanin synthesis and tyrosinase activity in α-MSH stimulated B16 cells in a dose-dependent manner ( P<0.05) . The melanin content and tyrosinase activity significantly decreased by 52.95% , 32.85% at 20 mg ·L-1of CMP, respectively. Treatment of 100 mg· L-1of arbutin reduced the melanin content and tyrosi- nase activity by 17.29% , 16.75% , respectively. Based on the results of this study, CMP showed a stronger anti-melanogenesis activity than that of positive control arbutin. After treated by CMP, the protein and mRNA levels of TYR, TRP-1, TRP-2 and MITF were significantly inhibited compared to the α-MSH group ( P<0.05) . Conclusions CMP could suppress the melanogenesis in α-MSH stimulated B16 cells, and its mechanism may be related to its regulation of the pro-tein and mRNA expressions of TYR, TRP-1, TRP-2 and MITF, and the inhibition of tyrosinase activity.关键词
桑白皮/多酚/B16 细胞/α-黑素细胞刺激素/黑色素/酪氨酸酶Key words
Cortex Mori/polyphenol/B16 cells/α-melanocyte-stimulating hormone/melanin/tyrosinase分类
医药卫生引用本文复制引用
吴永祥,毕淑峰,姜薇,崔谱,金有贞,金泰完..桑白皮多酚对B16细胞内黑色素生成的影响及其机制[J].中国药理学通报,2018,34(9):1296-1301,6.基金项目
安徽省高校自然科学研究重点项目(No KJ2017A398) （No KJ2017A398）
安徽省留学回国人员创新项目择优资助计划重点项目 ( No 2017srst1) （ No 2017srst1）
