昆虫学报2018，Vol.61Issue(7)：808-814,7.DOI:10.16380/j.kcxb.2018.07.007
沙葱萤叶甲成虫越夏期间糖类、蛋白及脂肪含量的变化
Change of carbohydrate, protein and lipid contents in Galeruca daurica (Coleoptera: Chrysomelidae) adults during oversummering
摘要
Abstract
[Aim]This study aims to clarify the characteristics of main physiological and biochemical change in Galeruca daurica adults at different oversummering stages so as to lay a foundation for revealing the oversummering mechanism. [Methods]The contents of total sugar, trehalose, glycogen, lipid and water in G. daurica adults at different oversummering stages (pre-oversummering, during oversummering and post-oversummering) were determined by anthrone-sulfuric acid and methanol-chloroform methods, respectively, and the total protein content was detected by Coomassie brilliant blue G-250 method.[Results]The contents of total sugar, water and total protein in G. daurica adults were significantly higher at the pre-oversummering stage (26. 81 μg/mg, 77. 47％ and 63. 17 μg/mg, respectively) and post-oversummering stage (26. 41-26. 85 μg/mg, 75. 29％-76. 65％ and 59. 53-64. 93 μg/mg, respectively) than during oversummering (18. 77-21. 14 μg/mg, 61. 50％-67. 20％ and 39. 82-52. 54 μg/mg, respectively), whereas the change trend of glycogen contents was the opposite, being8. 43 μg/mg at the pre-oversummering stage, 5. 91-6. 14 μg/mg at the post-oversummering stage and10. 18-11. 58 μg/mg during oversummering, respectively, and the content of lipid was significantly less at the post-summering stage (11. 48％-11. 65％) than at the pre-oversummering stage (42. 48％) and during oversummering (36. 05％-64. 79％). The contents of lipid and total protein decreased gradually in G. daurica adults during oversummering, while the water, total sugar and glycogen contents showed no obvious change. The trehalose content in G. daurica adults at different oversummering stages fluctuated greatly from 2. 66 μg/mg to 25. 31 μg/mg. [Conclusion] Lipid and glycogen are the main energy materials of G. daurica adults during oversummering.关键词
沙葱萤叶甲/越夏/滞育/糖类/糖原/海藻糖/蛋白/脂肪Key words
Galeruca daurica/oversummering/diapause/carbohydrate/glycogen/trehalose/protein/lipid分类
生物科学引用本文复制引用
陈龙,周晓榕,高利军,谭瑶,庞保平..沙葱萤叶甲成虫越夏期间糖类、蛋白及脂肪含量的变化[J].昆虫学报,2018,61(7):808-814,7.基金项目
国家自然科学基金项目(31760517) （31760517）
