昆虫学报2018，Vol.61Issue(7)：851-859,9.DOI:10.16380/j.kcxb.2018.07.012
昆虫病原线虫和噻虫嗪混用对韭蛆的杀虫效果及其体内保护酶和解毒酶活性的影响
Bioefficacy of the combined application of entomopathogenic nematodes and thiamethoxam and its effects on the protective and detoxification enzyme activities in Bradysia odoriphaga (Diptera: Sciaridae) larvae
摘要
Abstract
[Aim] Pathogenic organisms or chemical pesticides can induce the defense responses of insects, and the physiological adaptation of insects might differ under the combined application of pathogenic organisms and chemical pesticides. This study aims to investigate the effects of combinedapplication of entomopathogenic nematodes and thiamethoxam on Bradysia odoriphaga. [Methods]The LT50 value of the combined application of Steinernema feltiae SF-SN strain (Sf) (60 IJs/larva) and thiamethoxam (15 mg/L) against the 3 rd instar larvae of B. odoriphaga was determined with filter paper culture method in the laboratory, and its effects on the protein content of enzyme solution and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferases (GSTs) and acetylcholinesterase (AChE) in B. odoriphaga larvae at different time after treatment were assayed by biochemical analysis. [Results] The LT50 value (41. 05 h) of the combined application of Sf and thiamethoxam against the 3 rd instar larvae of B. odoriphaga decreased by about 126. 88 h and 31. 77 h, respectively, as compared with those in treatments with the respective single agents with (Sf LT50:167. 93 h) and thiamethoxam (LT50: 72. 82 h). During 24-72 h after treatment, the corrected mortality rates of B. odoriphaga larvae in the combined treatment group were significantly higher than those in treatments with the respective single agent, and reached the peak (96. 61％) at 72 h. The protein contents of enzyme solution in B. odoriphaga larvae in the combined treatment group at 12, 24 and 36 h after treatment increased by 13. 88％, 46. 87％ and 57. 99％, respectively, as compared with the control group, and were significantly higher than those in the control group, Sf treatment group and thiamethoxam treatment group. The activities of SOD, CAT, AChE and GTSs in larvae in the combined treatment group decreased by 47. 48％, 28. 73％, 71. 04％ and 29. 97％ at 24 h after treatment, and by 46. 34％, 42. 22％, 58. 37％ and 11. 87％ at 36 h, respectively, as compared with the control. The activities of the four enzymes in the combined treatment group were significantly lower than those in the control group, Sf treatment group and thiamethoxam treatment group at 24 h and 36 h after treatment. [Conclusion]The LT50 value of the combined application of Sf and thiamethoxam against the 3 rd instar larvae of B.odoriphaga is significantly lower than those in treatments with the respective single agent, and the combined treatment can significantly restrain the activities of SOD, CAT, AChE and GTSs in larvae.关键词
韭菜迟眼蕈蚊/昆虫病原线虫/芫菁夜蛾斯氏线虫SF-SN/噻虫嗪/酶活性Key words
Bradysia odoriphaga/entomopathogenic nematode/Steinernema feltiae SF-SN/thiamethoxam/enzyme activity分类
生物科学引用本文复制引用
武海斌,宫庆涛,陈珍珍,姜莉莉,公义,许永玉,孙瑞红..昆虫病原线虫和噻虫嗪混用对韭蛆的杀虫效果及其体内保护酶和解毒酶活性的影响[J].昆虫学报,2018,61(7):851-859,9.基金项目
山东省农业科学院青年科研基金(2015YQN30) （2015YQN30）
山东省自然科学基金项目(ZR2018PC019) （ZR2018PC019）
