生命科学研究2018，Vol.22Issue(4)：259-270,12.DOI:10.16605/j.cnki.1007-7847.2018.04.001
青鱼Viperin是一种抗病毒蛋白质
Viperin of Black Carp Mylopharyngodon piceus is an Antiviral Protein
摘要
Abstract
Viperin has been shown to be an antiviral protein against a wide range of DNA and RNA viruses in vertebrates. Herein, the viperin of black carp Mylopharyngodon piceus has been cloned and characterized. The full-length c DNA of black carp viperin (bc Viperin) consists of 1 828 nucleotides and the predicted bc Viperin protein contains 360 amino acids, which is composed of a less conserved N-terminal amphipathic α-helix, a central radical S-adenosylmethionine (SAM) and a highly conserved C-terminal region. bc Viperin m RNA has been constitutively detected in all the selected tissues of black carp, including the heart, liver, spleen, kid-ney, intestine, muscle, skin and gills. The m RNA level of bc Viperin increased in all these tissues after both GCRV and SVCV infections. q-PCR data demonstrated that bc Viperin m RNA level in Mylopharyngodon piceus fin (MPF) cells also increased in response to both GCRV and SVCV infections. Western-blot demonstrated that the relative molecular mass of bc Viperin is around 42 k D; and the immunofluorescence staining of both He La and EPC cells showed that bc Viperin is a cytosolic protein. In addition, EPC cells transfected with plasmid expressing bc Viperin presented obviously improved antiviral ability against both SVCV and GCRV. Taken together, these findings support the conclusion that bc Viperin is an antiviral protein functioning in the innate immunity of black carp.关键词
青鱼/viperin/天然免疫/鲤春病毒血症病毒(SVCV)/草鱼呼肠孤病毒(GCRV)Key words
black carp/viperin/innate immunity/spring viremia of carp virus (SVCV)/grass carp reovirus (GCRV)分类
生物科学引用本文复制引用
田彧,陈慧,周旻昱,李俊,肖俊,冯浩..青鱼Viperin是一种抗病毒蛋白质[J].生命科学研究,2018,22(4):259-270,12.基金项目
国家自然科学基金资助项目(81471963) （81471963）
湖南省研究生科研创新项目(CX2017B185) （CX2017B185）
The National Natural Science Foundation of China(81471963) （81471963）
Hunan Provincial Innovation Foundation for Postgraduate(CX2017B185) （CX2017B185）
