生命科学研究2018，Vol.22Issue(4)：271-276,6.DOI:10.16605/j.cnki.1007-7847.2018.04.002
氧化应激时Peroxiredoxin Ⅱ膜质转移对红细胞渗透脆性的影响
Effect of Transfer of Peroxiredoxin Ⅱ from Membrane to Cytosol on Osmotic Fragility of Erythrocytes in Oxidative Stress
摘要
Abstract
To investigate the effect of transfer of peroxiredoxin Ⅱ (Prx Ⅱ) from membrane to cytoplasm on erythrocyte osmotic fragility under oxidative stress, the change of erythrocyte osmotic fragility induced by H2O2 was examined, and the condition of Prx Ⅱ transfer from membrane to cytoplasm in erythrocytes and the expression of the membrane proteins band 3 and spectrin of red blood cells were detected by Western-blot.The results showed that the osmotic fragility of red blood cells was increased, and Prx Ⅱ was transferred from the erythrocyte membrane to cytoplasm during oxidative stress. At the same time, the expression of proteins that maintain the stability of red blood cell membrane and the integrity of the cytoskeletal structure and function was reduced. These results indicated that Prx Ⅱ in the erythrocytes could be transferred from the membrane to cytoplasm under oxidative stress, and this would lead to the decreased expression levels of band 3 and spectrin and the increased osmotic fragility of the red blood cells.关键词
红细胞/渗透脆性/氧化应激(OS)/peroxiredoxin Ⅱ (Prx Ⅱ)/膜质转移Key words
erythrocyte/osmotic fragility/oxidative stress (OS)/peroxiredoxin Ⅱ (Prx Ⅱ)/transfer from membrane to cytoplasm分类
生物科学引用本文复制引用
于佳斌,卢悦,张津松,韩英浩..氧化应激时Peroxiredoxin Ⅱ膜质转移对红细胞渗透脆性的影响[J].生命科学研究,2018,22(4):271-276,6.基金项目
2016年黑龙江省大学生省级创新训练计划项目(2011310049) （2011310049）
