生命科学研究2018，Vol.22Issue(4)：283-290,8.DOI:10.16605/j.cnki.1007-7847.2018.04.004
人KIAA0146基因及蛋白质的生物信息学分析
Bioinformatics Analysis of Human KIAA0146 Gene and Protein
摘要
Abstract
KIAA0146 is a scaffold protein of homologous recombinant protein complex BLM/RAD51. Current-ly, there are few KIAA0146-related experimental studies. The effective bioinformatics analyses were therefore performed for providing clues for further experimental study of KIAA0146. Several transcription start sites and promoters were found at the KIAA0146 gene. Besides, two Cp G islands were found on the flank of promoter (2 127～2 177 bp) and (3 127～3 177 bp), respectively. It was found that KIAA0146 was an unstable hydrophilic protein without signal peptide and transmembrane domain. It was therefore considered as neither a secreted protein nor a transmembrane protein. However, the KIAA0146 was considered as nuclear protein with nuclear localization signal"RARGSKRKR"at its N-terminal. The constructed 3 D structure of KIAA0146 showed that it contained a nucleic acid-binding domain. It was finally found that RAD51, ATM, RAD51 B, MRE11 A, BLM, DNA2, RMI1, BRCA1, BRCA2 and XRCC2 might interact with KIAA0146. In addition, KIAA0146 was con-sidered to involve into the biological processes including DNA damage response and repair, development and cell metabolism regulation through these interacting proteins. In conclusion, the analyses of gene structure, protein subcellular location, 3 D structure and potential molecular function of KIAA0146 provide important theories and new clues for further experimental study on this molecule.关键词
KIAA0146/同源重组修复/支架蛋白质/生物信息学Key words
KIAA0146/homologous recombination repair/scaffold protein/bioinformatics分类
生物科学引用本文复制引用
周飞,刘雪梅,彭仲特..人KIAA0146基因及蛋白质的生物信息学分析[J].生命科学研究,2018,22(4):283-290,8.基金项目
广东省自然科学基金资助项目(2017A030310606) （2017A030310606）
韩山师范学院博士启动项目(QD20170504) （QD20170504）
广东大学生科技创新培育专项资金资助项目(pdjha0320) （pdjha0320）
