生命科学研究2018，Vol.22Issue(4)：305-310,331,7.DOI:10.16605/j.cnki.1007-7847.2018.04.007
屎肠球菌降胆固醇作用的初步研究
Preliminary Study on Cholesterol Reduction by Enterococcus faecium
摘要
Abstract
High blood cholesterol is a serious threat to human health. The cholesterol-reducing rates of 22 Enterococcus faecium strains were screened by the saponification-colorimetric method in vitro, and were found to range from 85.93％ ～ 21.25％. Three strains with better bile salt tolerant activity were selected for further studies. Among them, the acid resistance of E. faecium SP5-6 L was poor while strains JS2 and ML13-5 were able to survive at p H 2.0 and p H 3.0 for 3 h and in 0.3％, 0.5％ and 1％ bile salt for 12 h without losing their viability. All three strains had a high activity of bile salt hydrolase. The cholesterol-re-ducing rate of strain ML13-5 was increased from 52.71％ to 68.01％ at 0.3％ bile salt. In addition, three strains had no hemolysis, but with a different sensitivity to ten kinds of antibiotics. This preliminary study on the mechanism of cholesterol reduction by E. faecium provides basis for its further utilization and for development of related functional foods and drugs.关键词
屎肠球菌/胆固醇/耐酸耐胆盐/抗药性/安全性Key words
Enterococcus faecium/cholesterol/acid and bile salt resistance/drug resistance/security分类
生物科学引用本文复制引用
吴边,柳陈坚,李雅迪,尚云,李晓然..屎肠球菌降胆固醇作用的初步研究[J].生命科学研究,2018,22(4):305-310,331,7.基金项目
昆明理工大学学科方向团队(14078326) （14078326）
云南省科技厅—昆明医科大学应用基础研究联合专项项目[2017FE468-(122)] （122）
