生命科学研究2018，Vol.22Issue(4)：320-325,6.DOI:10.16605/j.cnki.1007-7847.2018.04.009
几种肿瘤生物标志物研究方法及结果的比较
Comparison of Methods and Results of Several Tumor Biomarkers
摘要
Abstract
Biomarkers play an important role in the prevention, diagnosis and treatment of tumors. They can be studied through body fluids, tissues or cells of human or animal models. Body fluids include blood, urine, and so forth. Researchers always focus on blood and do not pay enough attention to urine. However, in theo-ry, urine is not regulated by the body's homeostasis mechanism. It should be able to reflect changes in the body caused by diseases earlier and more sensitively and is therefore a more desirable source of biomarkers.Different proteins can also be used as candidate markers from conditioned cell culture media, tissues or or-gan perfusates. Herein, the results and methods of screening for differential proteins as biomarkers in con-ditioned media of tumor cells, hepatic perfusates from the rat model, and urine were compared. Analyzing the advantages and disadvantages of these three samples provides clues for future marker research.关键词
生物标志物/细胞条件培养基/肝脏灌流液/尿液/大鼠模型/蛋白质组Key words
biomarker/cell cultures/liver perfusates/urine/rat model/proteome分类
生物科学引用本文复制引用
王婷,高友鹤..几种肿瘤生物标志物研究方法及结果的比较[J].生命科学研究,2018,22(4):320-325,6.基金项目
国家重点研发计划资助项目(2016 YFC1306300) （2016 YFC1306300）
国家重点基础研究发展计划资助项目(2013CB530805) （2013CB530805）
北京市自然科学基金资助项目(7173264, 7172076) （7173264, 7172076）
中央高校基本科研专项资金资助项目(2015KJJCB21) （2015KJJCB21）
北京市重点实验室建设基金资助项目(110651103) （110651103）
北京师范大学科研启动基金资助项目 （）
北京师范大学科研基金资助项目(11100704) （11100704）
