中国农业科技导报2018，Vol.20Issue(6)：47-54,8.DOI:10.13304/j.nykjdb.2017.0824
草甘膦抗性菌株的分离鉴定及其抗性基因的克隆
Isolation, Identification and Its Resistant Gene Cloning of a New Glyphosate-resistant Strain
摘要
Abstract
A 400 mmol/L glyphosate-resistant strain S1536 was isolated from the glyphosate polluted soil. The strain grew well in the medium with glyphosate concentration between 100 ～ 400 mmol/L. The optimal pH was 6.0, optimal growth temperature was 30℃, and had resistance to ampicillin. Taking S1536 genomic DNA as a template, this experiment carried out amplification and sequencing and gained 16 S rDNA sequence, which registration number was MG519831 in Gen Bank. Compared with the published nucleotide sequence of 16 S rDNA in NCBI (National Center for Biotechnology Information), S1536 showed 99％ homology with Pantoea sp. Multiple sequence comparison between S1536 and the model bacteria of Pantoeasp of 16 S rDNA was conducted by ClustalW. This strain showed high identity with Pantoea rodasii, reaching 99. 2％, so it was named as Pantoea rodasii S1536. The strain possessed higher glyphosate resistance and good adaptability to temperature and pH. It was a superior glyphosate tolerant strain. aroA sequence resistant to herbicide was obtained through whole genome sequencing and gene annotation, which provided a solid foundation for further identification of gene function and exploitation of the molecular mechanism of high glyphosate resistance of Pantoea rodasii S1536.关键词
草甘膦抗性/鉴定/生长特性/泛菌属Key words
glyphosate resistance/identification/growth characteristics/Pantoea sp.分类
生物科学引用本文复制引用
杨小艳,刘亚娟,吴红,王忠伟,雷开荣,谢树章..草甘膦抗性菌株的分离鉴定及其抗性基因的克隆[J].中国农业科技导报,2018,20(6):47-54,8.基金项目
重庆市农业科学院良种创新暨重大科技推广项目(NKY-2016AA003-2) （NKY-2016AA003-2）
重庆市基础科学与前沿技术研究一般项目(cstc2017jcyjAX0395) （cstc2017jcyjAX0395）
重庆市科研院所绩效激励引导专项(cstc2017jxjl80001)资助. （cstc2017jxjl80001）
