中国中西医结合杂志2018，Vol.38Issue(8)：963-968,6.DOI:10.7661/j.cjim.20180324.013
益肺逐积方联合环磷酰胺对Lewis肺癌小鼠肿瘤组织CD151、整合素α3及β1 mRNA表达水平的影响
Effect of Yifei Zhuji Formula Combined with Cyclophosphamide on CD151, ITG α3 and ITG β1 mRNA Expression in Tumor of Mice with Lewis Lung Cancer
摘要
Abstract
Objective To explore the effect of Yifei Zhuji Formula (YFZJF) combined with Cyclophosphamide (CTX) on CD151 and integrin (ITG) α3 and ITGβ1 mRNA expression in tumor tissue of mice with Lewis lung cancer.Methods After the mice model of lung cancer was established by subcutaneous inoculation of Lewis cells in the right forelimb, the C57 BL/6 mice were randomly divided into 4 groups, i.e., model group, CTX group, YFZJF group and combination group, 10 in each group.The mice in YFZJF group and model group were given YFZJF (14.95 g/kg) or normal saline respectively by gavage once a day.CTX group mice received CTX (40 mg/kg) at 72 h and the 10 th day after inoculation by intraperitoneal injection.Combination group were given CTX combined with YFZJF (14.95 g/kg) .The status of mice and tumor formation was observed.The mice were sacrificed after treatment for 14 days, the body mass and tumor volume were measured as well as the tumor inhibition rate was calculated.Tumor tissue pathological changes were observed by routine paraffin sectiontechnique.The expression of CD151, ITGα3 and ITGβ1 mRNA were determined by Real-time PCR.ResultsCompared with model group, the tumor volume was decreased significantly in YFZJF group and combination group (P＜0.05) , and the inhibitory rate order from high to low were combination group, YFZJF group, CTX group.Pathology results showed that tumor tissue arranged in flake shape and infiltrated into skeletal muscle, the tumor cells appeared the diffuse growth along the wall of bronchioles and alveolar.The expressions of CD151 mRNA in YFZJF, CTX and combination groups were decreased significantly compared with model group (P＜0.01, P＜0.05) , and ITGα3 mRNA in combination group was also decreased (P＜0.05) .Compared with CTX group, the expression of ITGβ1 mRNA in YFZJF group was decreased (P＜0.05) .Conclusion YFZJF combined with CTX treatments can suppress the growth of Lewis lung cancer effectively, and the effect of YFZJF may be involved in down-regulating the expression of CD151, ITGα3 and ITGβ1 mRNA in tumor tissue.关键词
益肺逐积方/环磷酰胺/Lewis肺癌小鼠模型/CD151基因/整合素α3/整合素β1Key words
Yifei Zhuji Formula/Cyclophosphamide/Lewis lung cancer mice/CD151/ITG α3/ITG β1引用本文复制引用
张娜,刘学芳,蒋时红,潘晓丽..益肺逐积方联合环磷酰胺对Lewis肺癌小鼠肿瘤组织CD151、整合素α3及β1 mRNA表达水平的影响[J].中国中西医结合杂志,2018,38(8):963-968,6.基金项目
河南省科技厅基础与前沿技术研究项目(No.152300410109) （No.152300410109）
郑州市科技局科技攻关项目(No.20150307) （No.20150307）
