中国中西医结合杂志2018，Vol.38Issue(8)：991-996,6.DOI:10.7661/j.cjim.20180626.183
马钱子总碱对兔膝骨关节炎模型软骨损伤修复作用及机制
Effect of Total Alkaloids from Semen Strychni on Cartilage Injury in Rabbit Model of Knee Osteoarthritis and It′s Mechanisms
摘要
Abstract
Objective To investigate the effect and mechanism of total alkaloids fromsemen strychni (TASS) in rabbit Hulth-Telhag model of knee osteoarthritis (OA) .Methods New Zealand rabbits were randomly divided into the normal control group, model group, sham operation group, high, medium and low dose TASS groups, and the sodium hyaluronate group, 8 in each group.With the exception of the normal control group, all groups received the Hulth-Telhag surgical procedure on knees to induce OA.Eight weeks after osteoarthritis modeling, high, medium and low dose TASS groups were administered with 0.3, 0.2, or 0.1 mL TASS injection into each joint cavity, respectively.In the sodium hyaluronate group, 0.2 mL of sodium hyaluronate was injected into each joint cavity.The treatment was given 2 times per week, for 5 consecutive weeks.New Zealand rabbits in the normal control group, model group, and sham operation group were conventionally fed, without other special treatment.One week after intervention, pathological changes of articular cartilage and Mankin′s score were observed by light microscopy.Hemorheology was used to detect whole blood viscosity (low shear, middle shear, high shear) and plasma viscosity.The levels of NO, SOD, and lipid peroxidase (LPO) in joint fluid and pyridol (PYD) in urine were measured by ELISA.Results Compared with the normal control group, the Mankin′s score of cartilage, whole-blood viscosity and plasmatic viscosity, the levels of NO, LPO and PYD were increased in the model group (P＜0.05) , while the SOD was decreased (P＜0.05) .Compared with the model group, the Mankin′s score of cartilage was decreased in the high and low dose TASS groups and sodium hyaluronate group (P＜0.05) ;whole-blood viscosity and plasmatic viscosity, the levels of NO, LPO and PYD in the synovial fluid decreased while the SOD was increased in the treatment groups (P＜0.05) .Compared with low dose TASS group, the medium dose TASS group exhibited an increased whole-blood viscosity and SOD levels (P＜0.05) , decreased synovial fluid levels of NO, LPO and PYD (P＜0.05) .Compared with medium dose TASS group, whole-blood and plasmatic viscosity, SOD levels were increased (P＜0.05) , NO, LPO and PYD levels in the synovial fluid were decreased in high dose TASS group (P＜0.05) .Compared with the sodium hyaluronate group, the Mankin′s score, NO, LPO and PYD levels were increased in the low dose TASS group (P＜0.05) , and SOD decreased (P＜0.05) ;the medium dose TASS group demonstrated decreased Mankin′s score and SOD (P＜0.05) , increased NO, LPO and PYD (P＜0.05) ;the high dose TASS group exhibited increased Mankin′s score, whole-blood viscosity and plasmatic viscosity (P＜0.05) .Conclusions The TASS treatment has a repairing function for cartilage injury in a model of osteoarthritis.The mechanism is likely related to inhibition of NO-mediated apoptosis of chondrocytes by the SOD pathway.Repair may also be mediated by the improvement of cartilage metabolism.关键词
马钱子总碱/兔膝骨关节炎Hulth-Telhag模型/作用机制Key words
total alkaloids fromsemen strychni/rabbit Hulth-Telhag model of knee osteoarthritis/mechanism引用本文复制引用
洪振强,高弘建,苏友新,徐志斌,吴子健,何俊君..马钱子总碱对兔膝骨关节炎模型软骨损伤修复作用及机制[J].中国中西医结合杂志,2018,38(8):991-996,6.基金项目
福建省科技厅重点项目(No.2012Y0039) （No.2012Y0039）
福建省自然科学基金项目(No.2017J01830) （No.2017J01830）
