康复学报2018，Vol.28Issue(3)：27-31,5.DOI:10.3724/SP.J.1329.2018.03027
三七总皂苷对载脂蛋白E基因敲除小鼠主动脉组织脂质沉积的影响
Effect of Total Saponins of Panax Notoginseng on Lipids Deposition of Apolipoprotein E-Knockout Mice
摘要
Abstract
Objective:To study the effect of total saponins of panax notoginseng (PNS) on high-fat diet induced lipids deposition on the aortic tissue of apolipoprotein E-knockout (Apo E-/-) mice. Methods:The atherosclerosis models were prepared with a high-fat diet in the Apo E-/-mouse model for 12 weeks. All Apo E-/-mice were randomly divided into three groups (model group, simvastatin group and total saponins group), and the wild type C57 BL/6 J mice were taked as the control group. Apo E-/-mice continuely intook high fat feeding by gavage for 8 weeks. Blood and the aortic root tissue were collected and serum lipid level was detected by biochemical analyzer, the serum IL-1β, IL-10 concentration was detected by ELISA, the lipid deposition of aortic tissue was observed by oil red O staining, CD+68 macrophage aggregation of aortic tissue was observed by immunohistochemical staining. Results:In the model group, TC and LDL-C were significantly increased, HDL-C was slightly reduced, and IL-1β, IL-10 were also increased significantly (P＜0.05). Aortic tissue oil red O positive staining cells increased significantly (P＜0.05), plaque lipid deposition was evident. The dots or patches protrude into the vascular and the vascular cavity was markedly narrowed, CD+68 macrophage was also increased significantly in aortic tissue (all P＜0.05). Compared to the model group, TC, LDL-C and IL-1β, IL-10 were significantly reduced (P＜0.05), HDL-C was slightly increased in both the simvastatin group and the total saponins group (P＜0.05). Positive staining cells of oil red O in aortic tissue, were significantly reduced, plaque lipid deposition and the area decreased. The degree of convexity was significantly reduced. CD+68 macrophage number and the macrophage aggregation were significantly reduced (all P＜0.05).Conclusion:PNS can regulate blood lipids, inhibit the immune inflammatory response, inhibit the lipid deposition of aortainhibiting and macrophage aggregation, which might be one of the anti-atherosclerosis mechanisms.关键词
动脉粥样硬化/脂质沉积/三七总皂苷/载脂蛋白E/基因敲除Key words
atherosclerosis/lipid deposition/total saponins of panax notoginseng/apolipoprotein E/knockout引用本文复制引用
付长庚,白瑞娜,刘玥,龙霖梓,陈可冀..三七总皂苷对载脂蛋白E基因敲除小鼠主动脉组织脂质沉积的影响[J].康复学报,2018,28(3):27-31,5.基金项目
国家自然科学基金青年基金项目 (81603483) （81603483）
中国中医科学院西苑医院苗圃课题项目[XYKY-MP (2013) -31] （2013）
