西北国防医学杂志2018，Vol.39Issue(7)：426-431,6.DOI:10.16021/j.cnki.1007-8622.2018.07.002
陕西省部分地区土拉弗朗西斯菌病自然疫源地调查研究
Investigation of natural epidemic focus of Francisella tularensis in Shaanxi province
摘要
Abstract
Objective:To investigate whether there is natural epidemic focus of Francisella tularensis in Shaanxi province.Methods:Tick samples were collected by molecular method.Small rodents were investigated by night trap and retrospective epidemiological investigation.The colloidal gold test strips were used for serological tests.The nested-PCR method was used for pathogenic molecular biology test.SPSS 9.0 software was used for analysis.Results:Fifteen species of ixodidae and 24 species of rodentia were collected in Fu County, Long County and Nanzheng County, especially the large number of Dermacentor, Haemaphysalis and Muridae.The 707 serum samples were collected from the residents of Fu County, Long County and Nanzheng County.The serum IgG antibody was detected in 49 samples, the total positive rate was 6.93％, indicating that the local population had different degrees of the natural infection of Francisella tularensis.A total of 1 117 ticks were detected from some of the three counties, and25 ticks were detected from the three species:D.daghestanicus, H.Japonica and H.concinna, the total positive rate was 2.24％.Among them, the positive rate of H.Japonica was the highest, reaching 15.39％ (χ2=20.91, P =0.01), indicating that the natural infection in ticks was existed.The local distribution of the small rodents such as S.dauricus, T.sibiricus, A.agrarius, M.musculus and R.norvegicus distributes in surrounding provinces had been confirmed as the bacteria host and disseminator by previous research, indicating that the small rodents also existed the natural infection.Ten cases were sequenced from 25 positive specimens, obtained nucleotide sequence and Francisella tularensis of B substrains sequence homologous to AF247690.2 100％.The copy No.9 of 6 different copies was predominantly (40.00％) in the SSTR9 region.The clustering analysis using MEGA 4.0 software showed that there were two major groups.Conclusion:The natural epidemic focus of Francisella tularensis is existed in Fu County, Long County and Nanzheng County of Shaanxi province.关键词
土拉弗朗西斯菌病/土拉弗朗西斯菌/自然疫源地/陕西省Key words
tularemia/Francisella tularensis/natural epidemic focus/Shaanxi province分类
医药卫生引用本文复制引用
郑龙..陕西省部分地区土拉弗朗西斯菌病自然疫源地调查研究[J].西北国防医学杂志,2018,39(7):426-431,6.基金项目
军队后勤科研计划项目课题 (CLZ12J007) （CLZ12J007）
