西北国防医学杂志2018，Vol.39Issue(7)：476-479,4.DOI:10.16021/j.cnki.1007-8622.2018.07.014
ELISA法抗HIV检测设置灰区的意义分析
Analysis of anti-HIV detection result and the setting of grey zone by ELISA
黄小娟 1仝非 1李晓东 1辛艳泓1
作者信息
- 1. 解放军兰州总医院输血科,甘肃 兰州 730050
- 折叠
摘要
Abstract
Objective:To explore the necessity of establishing grey zone in the detection of HIV antibody by ELISA, and to provide evidence for clinical blood test.Methods:The relationship between the test results of Western Blottest and Nucleic acid amplification test of179 volunteer donors′samples which were reactive to anti-HIV reagents (ELISA, grey zone) were statistically analyzed.All the volunteer donors′samples were collectted in the Lanzhou blood center of the PLA during 2011-2016.Results:A total of 179 specimens were detected by WB and 1 specimens was uncertainty, the band was gp160, and later follow-up found that the results was positive.37 specimens were detected by NAT and 1 specimens were positive, and the results of these two methods indicate that the positive specimen is not the same blood donor.Conclusion:There are two meanings in establishing grey zone in the detection of HIV antibody by ELISA.On the one hand, it can avoid leakage and reduce the probability of serious social adverseevents caused by the transmission of AIDS through blood transfusion, on the other hand, it also causes the waste of valuable blood resources due to human factors.关键词
HIV抗体/ELISA/WB/NAT/灰区Key words
HIV antibody/ELISA/WB/NAT/gray zone分类
医药卫生引用本文复制引用
黄小娟,仝非,李晓东,辛艳泓..ELISA法抗HIV检测设置灰区的意义分析[J].西北国防医学杂志,2018,39(7):476-479,4.
