中国输血杂志2018，Vol.31Issue(6)：599-602,4.DOI:10.13303/j.cjbt.issn.1004-549x.2018.06.006
血型血清学ABO亚型95例的分子机制研究
Molecular mechanism of 95 ABO subtype cases in Hebei region
尹志柱 1孙国栋 1董姗姗 1高洁 1高丽星1
作者信息
- 1. 邯郸市中心血站,河北 邯郸 056000
- 折叠
摘要
Abstract
Objective To study the molecular mechanism of the ABO blood group subtypes in 95 Chinese people.Methods Samples were collected during Nov, 2013-Oct, 2017 which are forward-reverse-typing inconsistent; serological tests were conducted on these samples and genotyping by sequence-specific primer polymerase chain reaction (PCR-SSP) was performed. The 6 th—7 th exon gene was sequenced. Some of the specimens were sequenced through the 1 st—7 th exon. Results A total of 95 cases (73 cases of first certificate and 22 family members) was confirmed by blood type serological method.The PCR-SSP and gene sequencing tests revealed that, in the 73 cases, 13 cases (17. 8％) were subtype A; 17 cases (23. 3％) were subtype B; 22 cases (30. 1％) were type B (A)-type, yielding a total detection rate of 71. 2％ (52/73). 18 cases of ABO subtype gene were detected in the 22 pedigree members. 21 of the 73 cases (28. 8％, 21/73) failed in ABO typing. 5 of the 21 cases (6. 8％, 5/73) was detected with O gene but expressed weak A or weak B antigen; In 4 cases of 22 family members, the ABO subtype gene was not detected, and a total of 25 cases (25/95) were reported. Conclusion The genotypes and phenotypes of ABO subtypes did not completely match, and the same genotype of blood group serotype corresponded to different genotypes, and the same genotype showed different serological results.关键词
ABO亚型/血型血清学/PCR-SSP,基因测序Key words
ABO subtype/blood group serology/PCR-SSP, gene sequencing分类
医药卫生引用本文复制引用
尹志柱,孙国栋,董姗姗,高洁,高丽星..血型血清学ABO亚型95例的分子机制研究[J].中国输血杂志,2018,31(6):599-602,4.
