中国中西医结合杂志2018，Vol.37Issue(11)：1315-1319,5.DOI:10.7661/j.cjim.20171018.393
二至天癸方对IVF-ET患者颗粒细胞凋亡的影响
Effect of Erzhi Tiangui Decoction on Granulosa Cells Apoptosis in IVF-ET Patients with Shen-qi Deficiency
摘要
Abstract
Objective To observe the effect of Erzhi Tiangui Decoction( EZTGD) on in vitro fertilization and embryo transfer(IVF-ET) patients with Shen-qi deficiency by intervention of p-Akt、PI3K、Bad protein in PI3K/Akt signaling pathway. Methods Totally 80 infertile patients with Shen-qi deficiency undergoing IVF-ET were randomly assigned to the EZTGD treatment group (40 cases, EZTGD combined with IVF-ET) and the placebo group (40 cases, placebo combined with IVF-ET),and another 40 famale volunteers due to spouse problem as the control group (placebo combined with IVF-ET) were recruited. All of them were treated from the day of gonadotropin-releasing hormone agonist (GnRH-a) down regulation to the human chorionic gonadotropin (HCG) injection day. The clinical pregnancy rate and the apoptosis rate of granulosa cells were observed. The expression of p-Akt、PI3K、Bad were detected by Western-blot. Results Compared with the control group,the protein expression of PI3K,p-Akt in the placebo group were decreased, the protein expression of Bad was increased (P ＜0. 05,P ＜0. 01). Compared with the placebo group,the protein expression of PI3K,p-Akt in granulosa cells of the EZTGD treatment group were increased,while the protein expression of Bad was decreased(P ＜0. 05,P ＜0. 01). Compared with the placebo group,the apoptosis rate of ovarian granulosa cells reduced and the clinical pregnancy rate increased in the EZTGD treatment group and the control group (P ＜0. 05, P ＜0. 01).Conclusion EZTGD may be through PI3K/Akt signal transduction pathway, down-regulation of Bad factor expression, thereby inhibiting granulosa cells apoptosis,so as to improve the quality of ootid and the outcome of IVF-ET patients with Shen-qi deficiency.关键词
二至天癸方/肾气虚/PI3K/Akt信号转导通路/体外受精-胚胎移植Key words
Erzhi Tiangui Decoction/Shen-qi deficiency/PI3K/Akt signal transduction pathway/in vitro fertilization and embryo transfer引用本文复制引用
连方,相珊..二至天癸方对IVF-ET患者颗粒细胞凋亡的影响[J].中国中西医结合杂志,2018,37(11):1315-1319,5.基金项目
国家自然科学基金资助项目(No. 81273790) （No. 81273790）
山东省自然科学基金资助项目(No. ZR2016HP29)通讯作者:连方,Tel:13706403122,E-mail:f_lian@163.com （No. ZR2016HP29）
