中国中西医结合杂志2018，Vol.37Issue(11)：1345-1350,6.DOI:10.7661/j.cjim.20170823.220
补肾活血饮对帕金森病伴发抑郁模型大鼠行为学及脑内5-HT、NE及NPY的影响
Effects of Bushen Huoxuo Decoction on Ethology, 5-HT,NE and NPY in Rats with Parkinson's Disease and Depression
摘要
Abstract
Objective To explore the influence of Bushen Huoxuo Decoction (BHD) on ethology, 5-HT, NE and NPY in rats with Parkinson's disease(PD) and depression (PDD). Methods The PD model was established by injuring 6-hydroxydopamine into substantia nigra (SSN), while the depression model was established by chronic unpredictable mild stress and separation. Seventy-five PDD model rats were randomly divided into 5 groups by random digital table method: the model group, the BHD group, the Fluoxetine group,the Levodopa and Benserazide Hydrochlo-ride group and the Fluoxetine + Levodopa and Benserazide Hydrochlo-ride group, 15 in each group. Another 20 normal rats were recruited as the normal group. The behavior of rats was observed by sucrose preference test, forced swimming test and tail suspension test. The content of 5-HT and NE in rats' brain were measured by HPLC-ECD. The content of NPY in rats' brain and serum were measured by radioimmunoassay. Results Compared with the normal group, the weight of rats in the model group decreased significantly before and after treatment (P ＜ 0.01). In the model group,the time of tail suspension and forced swimming were significantly prolonged, the sucrose preference rate was markedly reduced, the content of 5-HT and NE in rats' brain were diminished,the content of NPY in rats' brain and serum were decreased after treatment(P ＜0. 01). The rats in the BHD group, the Fluoxetine group and the Fluoxetine + Levodopa and Benserazide Hydrochlo-ride group gained more weight than the model group after treatment (P ＜ 0. 01 ). Compared with the model group,the immobility time of tail suspension and forced swimming were significantly reduced (P ＜0. 01), the sucrose preference rate were notably increased (P ＜0. 01 ), the contents of NPY in rats' brain and serum were markedly increased (P ＜ 0. 01 ), and the contents of 5-HT and NE in rats' brain were increased in the BHD group, the Fluoxetine group and the Fluoxetine + Levodopa and Benserazide Hydrochlo-ride group(P ＜0. 01, P ＜0. 05). The rats in the BHD group, the Fluoxetine group and the Fluoxetine + Levodopa and Benserazide Hydrochlo-ride group gained more weight than the Levodopa and Benserazide Hydrochlo-ride group after treatment (P ＜0.01 ). Compared with the Levodopa and Benserazide Hydrochlo-ride group, the immobility time of tail suspension and forced swimming were reduced (P ＜ 0. 05), the sucrose preference rate was increased (P ＜0. 05),the content of NPY in rats' brain and serum were significantly increased in the BHD group, the Fluoxetine group and the Fluoxetine + Levodopa and Benserazide Hydrochlo-ride group (P ＜0. 01). Conclusions BHD can promote the release of NPY, increase the content of 5-HT and NE in rats with PDD and improve the depressive behavior of PDD rats.关键词
帕金森/抑郁/补肾活血饮/5-羟色胺/去甲肾上腺素/神经肽YKey words
Parkinson's disease/depression/Bushen Huoxuo Decoction/5-hydroxytryptamine/norepinephrine/neuropeptide Y引用本文复制引用
王海明,杨明会,李绍旦,刘毅,李敏..补肾活血饮对帕金森病伴发抑郁模型大鼠行为学及脑内5-HT、NE及NPY的影响[J].中国中西医结合杂志,2018,37(11):1345-1350,6.基金项目
国家自然科学基金资助项目(No. 81273969) （No. 81273969）
