中国中西医结合杂志2018，Vol.37Issue(11)：1351-1355,5.DOI:10.7661/j.cjim.20170915.223
氧化苦参碱对高脂诱导胰岛素抵抗小鼠肝脏脂肪酸氧化的影响
Effect of Oxymatrine on Fatty Acid Oxidation in Hepatic of Fat-Induced Insulin Resistance Mice
摘要
Abstract
Objective To investigate the effects of oxymatrine on fatty acid oxidation in liver of high fat diet induced insulin resistance mice. Methods 72 ApoE-/mice fed with high fat diet for 16 weeks to construct insulin resistance model were randomly divided into the model group and Oxymatrine 25, 50, 100 mg/kg groups, 18 in each group. Eighteen C57BL/6J mice were recruited as the control group. Then the mice were administered with appropriate oxymatrine by gastrogavage for 8 weeks in each dose group of oxymatrine, equal volume of pure water to the control and model group. Hyper insulinemiceuglycemic clamp test was used to evaluate insulin resistance of mice, while the glucose infusion rate(GIR) was measured. The levels of free fatty acid (FFA) in the serum and hepatic tissues, FBG, TC, TG and FINS were detected,while the insulin resistance index (HOMA-IR) was calculated. The expression levels of fatty acid oxidation regulators [ peroxisome proliferator-activated receptor α (PPARa), cytochrome P4502E1 (CYP2E1), cytochrome P4504A1 (CYP4A10)], fatty acid oxidation rate-limiting enzyme [carnitine palmitoyl transferase 1 (CPT1),cytochrome oxidase (COX1 ) and solution uncoupling protein 2(UCP2)] in liver tissues were examined by Real-time reverse transcription PCR (qRT-PCR) and Western blot. Results Compared with the control group, the body weight, serum FBG,TC, TG, FINS, HOMA-IR value, serum and liver FFA content increased significantly (P＜0. 05),while GIR decreased in the model group (P ＜0. 05). The mRNA and protein expression levels of PPARα,CYP2A1,CYP4A10 and CPT1 decreased (P ＜0. 05), while COX1 and UCP2 increased in the model group (P ＜0. 05). Compared with the model group,the levels of TC,TG and HOMA-IR decreased in oxymatrine 25 mg/kg group (P ＜0. 05), while the levels of FBG,TC, TG, FINS and HOMA-IR increased in oxymatrine 50 mg/kg group (P ＜0. 05). The levels of weight,FBG,TC, TG, FINS and HOMA-IR decreased in oxymatrine 100 mg/kg group (P ＜0.05), while GIR increased in oxymatrine 50 and 100 mg/kg groups (P ＜0. 05). Compared with the model group, the mRNA and protein expression levels of PPARα,CYP2E1, CYP4A10 and CPT1 increased in all oxymatrine groups (P ＜0. 05),COX1 and UCP2 mRNA and protein expression decreased (P ＜0. 05),while the contents of FFA in serum and liver decreased (P ＜0. 05). Conclusion Oxymatrine can regulate the fatty acid oxidation in liver, thus improving high-fat induced insulin resistance in mice.关键词
氧化苦参碱/高脂饮食/胰岛素抵抗/脂肪酸氧化Key words
oxymatrine/high fat diet/insulin resistance/fatty acid oxidation引用本文复制引用
王超,张会欣,邢邯英,王杏,张哲..氧化苦参碱对高脂诱导胰岛素抵抗小鼠肝脏脂肪酸氧化的影响[J].中国中西医结合杂志,2018,37(11):1351-1355,5.基金项目
国家重点基础研究发展计划(973计划)项目(No. 2012CB518606) （973计划）
河北省重大医学科研项目(No. zd2013002,No. zd2013004) （No. zd2013002,No. zd2013004）
