中国中西医结合杂志2018，Vol.37Issue(11)：1361-1366,6.DOI:10.7661/j.cjim.20171011.238
左归丸联合干细胞治疗对化疗后卵巢早衰大鼠的影响
Effect of Combined Treatment of Zuogui Pill and Mesenchymal Stem Cells on Chemotherapy-induced Premature Ovarian Failure in Rats
摘要
Abstract
Objective To observe effect of combined treatment of Zuogui Pill (ZGP), prescription of nourishing Shen-Yin, and mesenchymal stem cells (MSCs) on chemotherapy-induced premature ovarian failure(POF) in rats. Methods Sixty rats were randomly divided into the blank group, model group, MSCs group, ZGP group and MSCs +ZGP group, 12 in each group. Apart from blank group, rats were daily administered with cyclophosphamide (Cytoxan, CTX) by intraperitoneal injection for 15 days to establish POF model. Then rats in MSCs group were administered with MSCs (3 ×106/mL,1 mL) by single tail vain injection and daily intragastrically administered with normal saline(2 mL/d) for 28 day. The rats in ZGP group were administered with a single injection of 1 mL normal saline, then were daily intragastrically administered with ZGP (3 g/kg) for 28 day. The rats in MSCs +ZGP group were administered with MSCs (3 ×106/mL,1 mL)by single tail vain injection, and then were daily intragastrically administered with ZGP(3 g/ kg) for 28 day. The rats in blank group and model group were administered with normal saline as above. The rats were weighted at different time, namely before, on the 14th and the 28th day of ZGP administration. The ovaries and uterus were weighted after ZGP administration. Pathological changes of ovary and uterus were observed by HE staining. The levels of estradiol (E2),follicle-stimulating hormone (FSH),and luteinizing hormone ( LH) in peripheral blood were determined by enzyme-linked immunosorbent assay(ELISA). Results Compared with the blank group, the rats' weight in the model group were lost significantly (P ＜0.01),which became much more serious with time going. While compared with the model group, the rats' weight in 3 treatment groups were increased on the 14th and the 28th day of ZGP administration(P ＜0. 01). Compared with model group, the numbers of mature follicle and luteal cells were increased,the endometrium was thickened and the glands were increased in all treatment groups, especially in the MSCs +ZGP group. Compared with the blank group, the weights of ovary and uterus in the model group were decreased (P ＜0. 01),the E2 level was down regulated, the FSH and LH levels were up regulated (P ＜0. 01). While after treatment,the weights of ovary and uterus were increased(P ＜0. 05, P ＜0. 01) in both ZGP group and MSCs +ZGP group, LH level in the MSCs +ZGP group was down regulated(P ＜0. 05); the level of E2 was increased, while that of FSH was decreased in 3 treatment groups(P ＜0. 05,P ＜0. 01), especially in the MSCs +ZGP group (P ＜0. 05). Conclusions Both ZGP and MSCs are effective on treating chemotherapy-induced POF by increasing the level of E2,and decreasing that of FSH and LH, and simultaneously inhibiting the apoptosis of ovarian granulosa cells. The better effect could be got using the combined therapy.关键词
左归丸/补肾养阴药/干细胞/卵巢早衰Key words
Zuogui Pill/prescription of nourishing Shen-Yin/mesenchymal stem cells/premature ovarian failure引用本文复制引用
吴倩,秦佳佳,杨静,梁嘉丽..左归丸联合干细胞治疗对化疗后卵巢早衰大鼠的影响[J].中国中西医结合杂志,2018,37(11):1361-1366,6.基金项目
国家自然科学基金资助项目(No. 81373805) （No. 81373805）
