中国中西医结合杂志2018，Vol.37Issue(11)：1367-1371,5.DOI:10.7661/j.cjim.20170225.224
左归丸对免疫性卵巢早衰模型小鼠卵巢GDF-9/Smad2信号转导的影响
Effect of Zuogui Pill on GDF-9/Smad2 Signal Transduction in Premature Ovarian Failure Model Mice
摘要
Abstract
Objective To observe the effect of Zuogui Pill (ZP) on growth differentiation factor-9(GDF-9)/Smad2 signal transduction in premature ovarian failure ( POF) model mice, and to study its mechanism for treating POF. Methods Totally 84 female Balb/c mice were randomly divided into the blank control group (n =12),the model group (n =24),the ZP group (n =24),the diethylstilbestrol group (n =24). The POF model was established by subcutaneously multi-point injecting zona pellucida 3(ZP3) in mice of all groups except the blank control group. ZP at the daily dose of 2. 7 g/kg was administered to mice in the ZP group by gastrogavage, once per day, 6 times per week for 4 or 8 successive weeks. Diethylstilbestrol solution at the daily dose of 0. 15 mg/kg was administered to mice in the diethylstilbestrol group by gastrogavage, once per day,6 times per week for 4 or 8 successive weeks. The ratio of ovarian growth follicles was observed. Protein expressions of GDF-9 and Smad2 in ovarian tissue were detected by immunohistochemical assay and Western blot. Results Compared with the blank control group, the ovarian growth follicle ratio, and protein expressions of GDF-9 significantly decreased in the model group (P ＜0. 05). Compared with the model group at the same time point, ovarian growth follicle ratio and GDF-9 protein expression significantly increased in the ZP group and the diethylstilbestrol group(P ＜0. 05). Smad2 protein expression increased in the ZP group at week 8 (P ＜0. 05).Conclusions The incidence of POF might be associated with insufficient expression of GDF-9 protein in ovarian tissue, hypofunction of GDF-9/Smad2 signal transduction pathway, which resulted in ineffectively recruited primordial follicles and follicular atresia. ZP could increase expression of GDF-9 protein possibly via regulating the follicle growth and development through GDF-9/Smad2 signal transduction pathway, thus playing a role in treating POF.关键词
卵巢早衰/左归丸/生长分化因子-9/Smad2蛋白/信号转导Key words
premature ovarian failure/Zuogui Pill/growth differentiation factor-9/Smad2 protein/signal transduction引用本文复制引用
朱玲,章雅琼,彭艳丽,任锦锦..左归丸对免疫性卵巢早衰模型小鼠卵巢GDF-9/Smad2信号转导的影响[J].中国中西医结合杂志,2018,37(11):1367-1371,5.基金项目
国家自然科学基金资助项目(No. 81674016) （No. 81674016）
广东省教育厅重点实验室2011年度开放基金(No. ZF201102) （No. ZF201102）
