国际医药卫生导报2018，Vol.24Issue(19)：2892-2895,4.DOI:10.3760/cma.j.issn.1007-1245.2018.19.004
CR3-TLR2合作体在小鼠巨噬细胞识别马尔尼菲蓝状菌中的作用
The role of complement receptor 3-Toll like receptor 2 collaboration on murine macrophage in recognition of Talaromyces marneffei
摘要
Abstract
Objective To evaluate the role of complement receptor 3-Toll like receptor 2 on murine macrophage in recognition of Talaromyces marneffei.Methods The siRNAs targeting interfere with CR3 and/ or TLR2 on mouse macrophage cell line RAW264.7,targeting down the expression of CR3,TLR2,CR3-TLR2,and then co-cultured with inactivated Talaromyces marneffei conidia.Phagocytosis rate was detected by flow cytometry,culture cytokine level was detected by ELISA.Results CR3,TLR2,CR3-TLR2 on RAW264.7 cells was interfered by siRNA,then co-cultured with inactivated Talaromyces marneffei conidia,all of the phagocytic rate decreased (P＜0.05),and the interfering with CR3+TLR2 was obvious (P＜0.05).Meanwhile,the cytokines secreted by macrophages decreased (P＜0.05).Conclusions In early stage of innate immunity,CR3-TLR2 collaboration on macrophage was one of the major pattern recognition receptors that participated recognition and mediated phagocytosis of Talaromyces marneffei.Thl-type cytokines and Th2-type cytokines of IL-2,IFN-γ,IL-4,IL-10 were involved in anti-infection immunity of macrophage resistance to Talaromyces marneffei.关键词
巨噬细胞/小鼠/补体受体3/Toll样受体2/马尔尼菲蓝状菌Key words
Macrophages/Mouse/Complement receptor 3/Toll like receptor 2/Talaromyces marneffei引用本文复制引用
胡永轩,周冼苡,戚欣雨,韩雪,毛祖豪,吴中..CR3-TLR2合作体在小鼠巨噬细胞识别马尔尼菲蓝状菌中的作用[J].国际医药卫生导报,2018,24(19):2892-2895,4.基金项目
广东省自然科学基金项目(2015A030310035 （）
2017A030313457)Natural Science Foundation Projects of Guangdong Province (2015A030310035 （）
2017A030313457) （）
