国际医药卫生导报2018，Vol.24Issue(19)：2940-2944,5.DOI:10.3760/cma.j.issn.1007-1245.2018.19.016
MicroRNA-618在食管癌中的表达及其对细胞功能的研究
The expression of miRNA-618 in esophageal cancer and the study on its effect on cellular function
王红梅 1孙奋勇 2张成 1梁恩瑜 1郑智明 1黄宪章1
作者信息
- 1. 510006 广州中医药大学第二附属医院检验医学部
- 2. 200072 上海第十人民医院检验科
- 折叠
摘要
Abstract
Objective To explore the role of miR-618 in the development of esophageal cancer by investigating its expression in the tissue samples of esophageal cancer and its effect on biological function in the cell line.Methods The expression of miR-618 in the esophageal cancer tissue and its paracancerous tissue were determined by fluorescent real-time quantitative PCR.The proliferation and cell cycle of the esophageal cancer cells-EC 109 were detected by MTT and flow cytometry,respectively.Results The expression of miR-618 in esophageal cancer tissue was significantly higher than that in its paracancerous tissue.After treated with miR-618 mimics for 48 hour,the percentage of EC 109 cells in G0/G 1 stage significantly decreased and the percentage of EC109 cells in S stage significantly increased (P＜0.05).After treated with miR-618 mimics for 72 hours,the number of EC 109 cell increased.Conclusions The expression of miR-618 is up-regulated in esophageal cancer patients.MiR-618 can stimulate the proliferation of the esophageal cancer cells and promotes the cells into S stage.Therefore,miR-618 may be closely related to the development of esophageal cancer and might act as an oncogene in esophageal cancer.关键词
食管癌/微小RNA/细胞增殖/细胞周期Key words
Esophageal cancer/microRNA/Cell proliferation/Cell cycle引用本文复制引用
王红梅,孙奋勇,张成,梁恩瑜,郑智明,黄宪章..MicroRNA-618在食管癌中的表达及其对细胞功能的研究[J].国际医药卫生导报,2018,24(19):2940-2944,5.
