中国兽医科学2018，Vol.48Issue(9)：1093-1101,9.DOI:10.16656/j.issn.1673-4696.2018.0177
捕食性真菌Duddingtonia flagrans线虫诱导发酵液的蛋白质组学分析
Analysis of the fermentation broth of Duddingtonia flagrans under conditions by proteomics
摘要
Abstract
In recent years,more and more attention had been paid to the nematode-trapping mechanism of Duddingtonia flagrans in biological control,and further analysis of the fermentation broth by proteomics is helpful to reveal the biochemical mechanism of D. flagrans to kill nematode. The mass spectrometry-based non-labeled quant itary technique was firstly used to study the proteomics of fermentation broth of induced or not from D. flagrans. In result,the total number of proteins identified was 258, and there were 46 proteins expressed differentially in the two tested groups. When analyzed by GO annotation,the differently expressed proteins had various functions of proteins in many biological processes in the fermentation broth induced by Strongylus sp. Besides,they were also involved in catalytic and binding activities,and the processes of sensory environmental stimuli,signal transduction, peroxidase activity,cell wall biogenesis and so on.Further more,compared with the control group,catalase and methyl citrate synthase precursor in tricarboxylic acid cycle were obviously increased and accelerated aerobic respiration,which predicting that D. flagrans secretes extracellular proteases that also needed oxidative stress. The differential expression of serine enzymes and tyrosinaseprecursor may be an important virulence factor of D. flagrans infecting nematodes. KEGG analysis indicated that these proteins were involved in synthesis and metabolism of glycoge, lipids and animo acids and their secondary metabolites. Therefore,it is of great significance to study proteins produced by the nematode-trapping fungi in the process of trapping and killing nematodes,and it would be of great help for the use of the nematode-trapping fungi to control parasitic nematode disease in future.关键词
捕食性真菌/Duddingtonia flagrans/发酵液/非标记定量蛋白质组学Key words
nematode-trapping fungi/Duddingtonia flagrans/fermentation broth/label-free proteomics分类
农业科技引用本文复制引用
梁萌,郭少平,陈湘江,杨晓野,王瑞,陈林军,王新芳,尹相吉,聂福贵,李莹莹,郭宏年,王文蕊,钱英红..捕食性真菌Duddingtonia flagrans线虫诱导发酵液的蛋白质组学分析[J].中国兽医科学,2018,48(9):1093-1101,9.基金项目
国家重点研发计划项目(2017YFD0501200) （2017YFD0501200）
国家自然科学基金项目(31460656) （31460656）
内蒙古自然科学基金项目(2014MS0339,2015MS0308) （2014MS0339,2015MS0308）
家畜疫病病原生物学国家重点实验室开放基金项目(SKLVEB2017KFKT010, SKLVEB2015KFKT013) （SKLVEB2017KFKT010, SKLVEB2015KFKT013）
