中国兽医科学2018，Vol.48Issue(9)：1109-1115,7.DOI:10.16656/j.issn.1673-4696.2018.0168
一株猪源肺炎克雷伯杆菌的分离与鉴定
Isolation and identification of a strain of Klebsiella pneumoniae from pig
摘要
Abstract
A suspected Klebsiella strain was isolated from pork by chance in our laboratory. In order to identify the suspected bacteria,the suspected bacteria were analyzed by means of bacterial isolation and culture,physicochemical properties, 16S rRNA sequence analysis,PCR amplification and bacterial pathogenicity test. The antibiotic sensitivity test results showed that the isolate was highly sensitive to norfloxacin,chloramphenicol and gentamicin,and was resistant to penicillin and cefalexin. In addition,the results of the urea decomposition test and citrate utilization test were positive,and the results of the hydrogen sulfide test and indole test were negative. The 16S rRNA amplification sequence was compared with the nucleotide sequence of Klebsiella pneumoniae published in GenBank. The homology of the isolated strain and Klebsiella acid was from 40. 1％ to 47. 2％,and the nucleotide homology of Klebsiella pneumoniae was from 86. 8％ to 99. 9％ . The isolated strain was confirmed as Klebsiella pneumoniae and named as GZAS2016. PCR amplification of the phoE gene fragment showed a 368 bp band,which further confirmed that the isolate was Klebsiella pneumoniae. In the animal pathogenicity test,the clinical symptoms of SPF mice began to appear at hour 2 after inoculation,and died within 12—15 h. It was proved that the isolated strains had certain pathogenicity to mice. The above-mentioned results showed that a pathogenic Klebsiella pneumoniae strain GZAS2016 was isolated from pork.关键词
肺炎克雷伯杆菌/理化特性/PCR扩增/16S rRNA/致病性Key words
Klebsiella pneumonia/physicochemical properties/PCR amplification/16S rRNA/pathogenicity分类
农业科技引用本文复制引用
徐丽,温贵兰,张升波,李昌红,林汉卿,田浪,杨佰启..一株猪源肺炎克雷伯杆菌的分离与鉴定[J].中国兽医科学,2018,48(9):1109-1115,7.基金项目
贵州省科学技术基金项目[黔科合J字(2015)2046号] （2015）
国家自然科学基金项目(31460668) （31460668）
