中国兽医科学2018，Vol.48Issue(9)：1116-1123,8.DOI:10.16656/j.issn.1673-4696.2018.0180
新疆牛蓝舌病毒的分离和鉴定
Isolation and identification of bluetongue virus from cattle in Xinjiang
摘要
Abstract
To monitor the prevalence of bluetongue in the natural environment in Xinjiang and to isolate the blue-tongue virus(BTV),in this study,we set ten cattle as sentinel animals in Yuli County, Bazhou. Sentinel animal serum and anticoagulant were collected weekly from May to November,2016,and 280 samples were collected respectively. All serum samples were subjected to BTV antibody detection by the competitive ELISA (c-ELISA) method. All anticoagulated blood samples were inoculated into C6/36, BHK21,and Vero cells. The cells were blindly transmitted for 5 generations and 4 cells developed lesions. The BTV antigen capture ELISA(Ac-EL ISA) was performed on these cell fluids,and the BTV serogroup-specific fragment VP7 gene was identified by PCR,and the immunoelectron microscopy was performed. In result,all of the cytopathogenic samples were positive for BTV antigen detection. The target gene fragment of 1 156 bp was obtained by RT-PCR. Bluetongue virus particles were observed by the immunoelectron microscopy.In conclusion,this study successfully isolated cattle source BTV in Xinjiang for the first time.关键词
牛/蓝舌病毒/分离/鉴定/RT-PCRKey words
cattle/bluetongue virus/isolation/identification/RT-PCR分类
农业科技引用本文复制引用
丁梦玥,孟肖潇,马俊杰,谷文喜,钟旗,马晓菁,叶锋,刘帅,薛新梅,宋迎春,加帕尔·哈斯木,易新萍,刘丽娅..新疆牛蓝舌病毒的分离和鉴定[J].中国兽医科学,2018,48(9):1116-1123,8.基金项目
国家公益性行业项目(农业)专项资助项目(201303035) （农业）
